Department of Genome Regulation, Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany; Institute of Chemistry and Biochemistry, Freie Universität Berlin,14195 Berlin, Germany. Electronic address: https://twitter.com/@adrianobolondi.
Department of Genome Regulation, Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany. Electronic address: https://twitter.com/@helenekretzmer.
Curr Opin Genet Dev. 2022 Oct;76:101947. doi: 10.1016/j.gde.2022.101947. Epub 2022 Jul 12.
The totipotent zygote gives rise to diverse cell types through a series of well-orchestrated regulatory mechanisms. Epigenetic modifiers play an essential, though still poorly understood, role in the transition from pluripotency towards organogenesis. However, recent advances in single-cell technologies have enabled an unprecedented, high-resolution dissection of this crucial developmental window, highlighting more cell-type-specific functions of these ubiquitous regulators. In this review, we discuss and contextualize several recent studies that explore epigenetic regulation during mouse embryogenesis, emphasizing the opportunities presented by single-cell technologies, in vivo perturbation approaches as well as advanced in vitro models to characterize dynamic developmental transitions.
全能的受精卵通过一系列精心协调的调控机制产生多种细胞类型。表观遗传修饰物在从多能性向器官发生的转变中起着至关重要但仍未被充分理解的作用。然而，单细胞技术的最新进展使得对这个关键发育窗口进行前所未有的高分辨率剖析成为可能，突出了这些普遍存在的调控因子在更多细胞类型特异性功能上的作用。在这篇综述中，我们讨论并将最近的几项研究置于背景下，这些研究探讨了小鼠胚胎发生过程中的表观遗传调控，强调了单细胞技术、体内扰动方法以及先进的体外模型在描述动态发育转变方面带来的机遇。
