RASSF8-AS1 在结直肠癌中表达水平较低，上调 RASSF8 可抑制细胞侵袭和迁移。

RASSF8-AS1 displays low expression in colorectal cancer and up-regulates RASSF8 to suppress cell invasion and migration.

机构信息

Department of Anorectal, Xi'an Hospital of TCM, No.69, Fengcheng 8 Road, Xi'an, Shaanxi 710021, China.

Department of Traditional Chinese Medicine, Shaanxi University of Chinese Medicine, Xianyang, China.

出版信息

Pathol Res Pract. 2022 Sep;237:153996. doi: 10.1016/j.prp.2022.153996. Epub 2022 Jun 30.

DOI:10.1016/j.prp.2022.153996

PMID:35839610

Abstract

BACKGROUND

Colorectal cancer (CRC) is among the most prevalent cancers. Long non-coding RNAs (lncRNAs) are important participant in various cancers. Based on the literature, lncRNA RASSF8-AS1 inhibits laryngeal squamous cell carcinoma (LSCC) malignant progression. However, the role of RASSF8-AS1 in CRC remains unclear.

PURPOSE

This study centered on uncovering the role of RASSF8-AS1 and its related regulatory mechanisms in CRC cells.

METHODS

RT-qPCR and western blot were performed to examine the expression of target genes. Functional assays were conducted to determine the effect of target genes on the migration and invasion of CRC cells. Mechanism assays were also carried out to figure out the specific downstream mechanisms of RASSF8-AS1. In vivo assays were also involved.

RESULTS

The expression of RASSF8-AS1 and RASSF8 was positively correlated in CRC, and the two genes were down-regulated in CRC cells and tissues. Moreover, CRC cell invasion and migration as well as xenograft CRC tumor growth suppressed by RASSF8-AS1 overexpression were entirely recovered by RASSF8 knockdown or partially rescued by miR-33a-5p augment. As for the downstream mechanism, RASSF8-AS1 sponged miR-33a-5p to up-regulate RASSF8, or recruited HNRNPC to stabilize RASSF8 mRNA.

CONCLUSION

RASSF8-AS1 modulates miR-33a-5p/HNRNPC/RASSF8 axis to further impede CRC cell invasion and migration.

AVAILABILITY OF DATA

The research data is confidential.

摘要

背景

结直肠癌（CRC）是最常见的癌症之一。长链非编码 RNA（lncRNA）是各种癌症的重要参与者。根据文献，lncRNA RASSF8-AS1 抑制喉鳞状细胞癌（LSCC）恶性进展。然而，RASSF8-AS1 在 CRC 中的作用尚不清楚。

目的

本研究旨在揭示 RASSF8-AS1 及其在 CRC 细胞中的相关调节机制的作用。

方法

通过 RT-qPCR 和 Western blot 检测靶基因的表达。功能测定用于确定靶基因对 CRC 细胞迁移和侵袭的影响。机制测定还进行了以找出 RASSF8-AS1 的具体下游机制。还进行了体内测定。

结果

RASSF8-AS1 和 RASSF8 的表达在 CRC 中呈正相关，并且这两个基因在 CRC 细胞和组织中下调。此外，RASSF8-AS1 过表达抑制的 CRC 细胞侵袭和迁移以及异种移植 CRC 肿瘤生长完全被 RASSF8 敲低恢复，或部分被 miR-33a-5p 增强恢复。至于下游机制，RASSF8-AS1 海绵 miR-33a-5p 以上调 RASSF8，或募集 HNRNPC 以稳定 RASSF8 mRNA。