Schäfer R, Geisse S, Willecke K
J Cell Biochem. 1987 May;34(1):31-8. doi: 10.1002/jcb.240340105.
The transcriptional activity of ten cellular oncogenes was analyzed in somatic cell hybrids that had been obtained after fusion of tumorigenic Chinese hamster cells and normal mouse fibroblasts. The hybrids showed either the tumorigenic or the nontumorigenic phenotype (suppression of tumorigenicity). Out of ten c-onc genes analyzed, four (c-Ha-ras, c-Ki-ras, c-myc, and c-fos) were found to be transcriptionally active at similar levels in tumorigenic as well as in nontumorigenic (suppressed) hybrids. Thus we conclude that suppression of tumorigenicity in Chinese hamster X mouse somatic cell hybrids does not correlate with quantitative changes in expression of these cellular oncogenes. The remaining six cellular oncogenes (c-abl, c-erb A and B, c-fes, c-myb, and c-sis) were not transcriptionally active in these hybrids.
在由致瘤性中国仓鼠细胞与正常小鼠成纤维细胞融合后获得的体细胞杂种中，分析了十种细胞癌基因的转录活性。这些杂种表现出致瘤或非致瘤表型（致瘤性抑制）。在所分析的十个c-onc基因中，发现四个（c-Ha-ras、c-Ki-ras、c-myc和c-fos）在致瘤性杂种以及非致瘤性（抑制的）杂种中以相似水平转录活跃。因此我们得出结论，中国仓鼠×小鼠体细胞杂种中致瘤性的抑制与这些细胞癌基因表达的定量变化无关。其余六个细胞癌基因（c-abl、c-erb A和B、c-fes、c-myb和c-sis）在这些杂种中不转录活跃。
