Nedel Wagner L, Rodolphi Marcelo S, Strogulski Nathan R, Kopczynski Afonso, Montes Thiago H M, Abruzzi Jose, Portela Luis V
Intensive Care Unit - Hospital Nossa Senhora da Conceição, Grupo Hospitalar Conceição, Porto Alegre, RS, Brazil; Programa de Pós-Graduação em Bioquímica, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil.
Laboratory of Neurotrauma and Biomarkers, Departamento de Bioquímica, Programa de Pós-Graduação em Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil; Programa de Pós-Graduação em Bioquímica, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil.
Mitochondrion. 2022 Sep;66:7-12. doi: 10.1016/j.mito.2022.07.001. Epub 2022 Jul 14.
Antibiotics may trigger alterations in mitochondrial function, which has been explored in cells culture, and in animal model of sepsis. This study sought to evaluate whether antibiotic therapy affects mitochondrial bioenergetics in a 68-patients clinical study. We studied mitochondrial respiratory rates at two time points: the first day of antibiotic administration and three days after. The Δbasal, ΔCI, ΔCII respiration, and ΔBCE respiratory rates were not different between patients administered with polymyxin, vancomycin, amoxicillin-clavulanate, and azithromycin compared to those who were not administered. Specific beta-lactams are associated with specific modifications in mitochondrial respiratory endpoints - patients who used meropenem had higher delta C2 values compared to those who did not (p = 0.03). Patients who used piperacillin-tazobactam had lower delta C1 (p = 0.03) values than those who did not, but higher delta C2 values (p = 0.02). These mitochondrial metabolic signatures in isolated lymphocytes challenges the proposed effects of antibiotics in mitochondrial bioenergetics of cell cultures, but at current status have an uncertain clinical significance.
抗生素可能会引发线粒体功能的改变，这一点已在细胞培养和脓毒症动物模型中得到研究。本研究旨在通过一项纳入68名患者的临床研究，评估抗生素治疗是否会影响线粒体生物能量学。我们在两个时间点研究了线粒体呼吸速率：抗生素给药的第一天和三天后。与未接受多粘菌素、万古霉素、阿莫西林 - 克拉维酸和阿奇霉素治疗的患者相比，接受这些药物治疗的患者的基础呼吸速率变化（Δbasal）、复合物I呼吸速率变化（ΔCI）、复合物II呼吸速率变化（ΔCII）以及呼吸控制率变化（ΔBCE）并无差异。特定的β - 内酰胺类药物与线粒体呼吸终点的特定改变相关——与未使用美罗培南的患者相比，使用美罗培南的患者的C2变化值更高（p = 0.03）。与未使用哌拉西林 - 他唑巴坦的患者相比，使用该药物的患者的C1变化值更低（p = 0.03），但C2变化值更高（p = 0.02）。分离淋巴细胞中的这些线粒体代谢特征对细胞培养中线粒体生物能量学方面抗生素的假定作用提出了挑战，但就目前情况而言，其临床意义尚不确定。
