College of Traditional Chinese Medicine, Hebei University, Baoding, China.
Baoding NO.2 Hospital, Baoding, China.
Anim Genet. 2022 Oct;53(5):685-689. doi: 10.1111/age.13244. Epub 2022 Jul 17.
Sno-lncRNAs are intron-derived long noncoding RNAs (lncRNAs) with snoRNA ends. Sno-lncRNAs were first discovered in the human Prader-Willi (PWS)/Angelman (AS) imprinted domain. Here, we report the identification and characterization of four sno-lncRNA types (sno-lncRNA1, sno-lncRNA2, sno-lncRNA3, and sno-lncRNA4) in the bovine PWS/AS imprinted domain. Reverse transcription-PCR first determined the cDNA sequences of the four bovine sno-lncRNAs. A gene structure analysis showed that sno-lncRNA1 lacks introns, but sno-lncRNA2 and sno-lncRNA3 have one and two introns respectively. The three sno-lncRNAs have similar snoRNA ends. Moreover, the three have similar snoRNAs at their 5' and 3' ends. The head-to-tail orientation has six sno-lncRNA copies arranged between bovine SNORD116-6 and SNORD116-12. Moreover, only a copy of sno-lncRNA4 was located between SNORD116-3 and SNORD116-4. The expression of the four sno-lncRNAs was analyzed in the bovine heart, liver, spleen, lung, kidney, muscle, fat, brain, and placenta tissues. The monoallelic expression of sno-lncRNA4 was determined in bovine tissues. The results showed that the four sno-lncRNAs are widely expressed in the nine tissues, although sno-lncRNA3 and sno-lncRNA4 were undetected in the placenta. Moreover, an informative single nucleotide polymorphism (rs448706424) revealed the allelic expression of sno-lncRNA4 in exon 2 of sno-lncRNA4. The bovine genome had six copies of sno-lncRNA1, sno-lncRNA2, and sno-lncRNA3, but their allelic expression was not identified.
Sno-lncRNAs 是具有 snoRNA 末端的内含子衍生的长非编码 RNA（lncRNA）。 sno-lncRNA 最初在人类 Prader-Willi（PWS）/Angelman（AS）印迹域中被发现。在这里，我们报告了在牛 PWS/AS 印迹域中鉴定和表征的四种 sno-lncRNA 类型（ sno-lncRNA1、 sno-lncRNA2、 sno-lncRNA3 和 sno-lncRNA4）。 反转录-PCR 首先确定了这四种牛 sno-lncRNA 的 cDNA 序列。 基因结构分析表明 sno-lncRNA1 缺乏内含子，但 sno-lncRNA2 和 sno-lncRNA3 分别具有一个和两个内含子。 这三个 sno-lncRNA 具有相似的 snoRNA 末端。 此外，它们在 5'和 3'端具有相似的 snoRNA。 头对头方向在牛 SNORD116-6 和 SNORD116-12 之间排列有六个 sno-lncRNA 拷贝。 此外，只有 sno-lncRNA4 的一个拷贝位于 SNORD116-3 和 SNORD116-4 之间。 在牛心脏、肝脏、脾脏、肺、肾脏、肌肉、脂肪、大脑和胎盘组织中分析了这四个 sno-lncRNA 的表达。 在牛组织中确定了 sno-lncRNA4 的单等位基因表达。 结果表明，这四个 sno-lncRNA 在九个组织中广泛表达，尽管 sno-lncRNA3 和 sno-lncRNA4 在胎盘中未检测到。 此外，一个信息丰富的单核苷酸多态性（rs448706424）揭示了 sno-lncRNA4 在 sno-lncRNA4 外显子 2 中的等位基因表达。 牛基因组有六个 sno-lncRNA1、 sno-lncRNA2 和 sno-lncRNA3 的拷贝，但它们的等位基因表达尚未确定。
