Song M, Liu Y, Wang X J, Zhang L W, Liu Q, Chen T F, Su X, Li W W, Lyu L X, Yang Y F
Department of Psychiatry, the Second Affiliated Hospital of Xinxiang Medical University, Henan Mental Hospital, Henan Key Lab of Biological Psychiatry, Xinxiang 453002, China.
Zhonghua Yi Xue Za Zhi. 2022 Jul 19;102(27):2108-2114. doi: 10.3760/cma.j.cn112137-20211125-02631.
To investigate the association of single nucleotide polymorphisms (SNP) of glutamate receptor metabotropic 5 (GRM5) gene with schizophrenia susceptibility(SZ) in a Chinese Han population. Twenty-two SNPs located in GRM5 gene in 528 paranoid SZ patients and 528 control subjects recruited from northern Henanwere analyzed. The clinical features of 267 first-episode SZ patients were assessed with the Positive and Negative Syndrome Scale (PANSS). The SZ group included 264 males and 264 females, aged (27±8) years; the healthy control group had 264 males and 264 females, aged (28±8) years.The differences in the genotypic and allelic frequencies of two SNPs (rs567990 and rs12421343) were statistically significant between the SZ patients and control groups (all <0.05). The allele frequency of rs504183 was also statistically different between the two groups (=0.030). When the subjects were stratified by sex, the genotypic and allelic frequencies of rs12421343 in female subjects were statistically different between the SZ patients and control groups. The allele frequencies of SNPs (rs12422021, rs567990, and rs7101540) were also statisticallydifferent between the two groups (all <0.05). Meanwhile, rs567990 AG+GG carriers had a higher risk for SZ than AA carriers in female subjects(=1.946, 95%: 1.264-2.995). In addition, the patients with different genotypes (GG, AA+AG) of rs12422021 showed statistically significant differences in PANSS total score(84.8±24.4 vs 75.3±18.6), positive (16.2±4.3 vs 14.4±4.2), excitement (12.4±5.1 vs 10.2±4.1) and cognitive impairment factor scores (15.2±6.8 vs 13.3±3.9) (all <0.05). The patients with AC and the other two genotypes (AA and CC) of rs504183 showed statistically significant differences in PANSS negative factor score(27.4±9.9 vs 24.7±8.4 and 23.4±8.1, both <0.05). The current study provides further evidence that GRM5 is associated with SZ, and suggests a putative sex difference.
探讨中国汉族人群中代谢型谷氨酸受体5（GRM5）基因单核苷酸多态性（SNP）与精神分裂症易感性（SZ）的关联。对从豫北地区招募的528例偏执型SZ患者和528例对照者中位于GRM5基因的22个SNP进行分析。用阳性和阴性症状量表（PANSS）评估267例首发SZ患者的临床特征。SZ组包括264例男性和264例女性，年龄（27±8）岁；健康对照组有264例男性和264例女性，年龄（28±8）岁。SZ患者与对照组之间两个SNP（rs567990和rs12421343）的基因型和等位基因频率差异有统计学意义（均<0.05）。两组间rs504183的等位基因频率也有统计学差异（=0.030）。按性别分层后，女性SZ患者与对照组之间rs12421343的基因型和等位基因频率有统计学差异。SNP（rs12422021、rs567990和rs7101540）的等位基因频率在两组间也有统计学差异（均<0.05）。同时，在女性受试者中，rs567990的AG + GG携带者患SZ的风险高于AA携带者（=1.946，95%：1.264 - 2.995）。此外，rs12422021不同基因型（GG、AA + AG）的患者在PANSS总分（84.8±24.4 vs 75.3±18.6）、阳性（16.2±4.3 vs 14.4±4.2）、兴奋（12.4±5.1 vs 10.2±4.1）和认知损害因子评分（15.2±6.8 vs 13.3±3.9）方面差异有统计学意义（均<0.05）。rs504183的AC与其他两种基因型（AA和CC）的患者在PANSS阴性因子评分方面差异有统计学意义（27.4±9.9 vs 24.7±8.4和23.4±8.1，均<0.05）。本研究提供了进一步证据表明GRM蛋白5与精神分裂症有关，并提示可能存在性别差异。
