用于福赛斯坦纳菌的肉汤培养基的研制。
Development of a broth medium for Bacteroides forsythus.
作者信息
Dzink J L, Smith C M, Socransky S S
出版信息
J Clin Microbiol. 1987 May;25(5):925. doi: 10.1128/jcm.25.5.925-.1987.
Abstract
Bacteriodes forsythus has been implicated as a possible pathogen in certain destructive periodontal diseases. This species has been extremely difficult to grow in broth, thus hampering its characterization and identification. A broth medium was developed consisting of 30 g of phytone, 10 g of NaCl, and 1 g of filter-sterilized L-cysteine per liter. The medium supported the growth of 23 fresh isolates of B. forsythus from six subjects to optical densities averaging 0.71 +/- 0.20 (standard deviation) at 550 nm after 48 to 72 h of anaerobic incubation.
摘要
福赛斯坦纳菌被认为是某些破坏性牙周疾病的可能病原体。该菌种在肉汤中极难生长，因此阻碍了对其特性的描述和鉴定。开发了一种肉汤培养基，每升含有30克植物蛋白胨、10克氯化钠和1克经滤膜除菌的L-半胱氨酸。在厌氧培养48至72小时后，该培养基支持从六个受试者中分离出的23株新鲜福赛斯坦纳菌的生长，在550纳米处的光密度平均为0.71±0.20（标准差）。
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