Xie Yihui, Xu Ziqian, Shi Weimin, Mei Xingyu
Department of Dermatology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Regen Ther. 2022 Jul 3;21:148-156. doi: 10.1016/j.reth.2022.06.007. eCollection 2022 Dec.
A large number of autologous melanocytes are required for surgical treatment of depigmentation diseases such as vitiligo. The purpose of this experiment is to explore the application of melanocytes induced by mesenchymal stem cells to clinical treatment. Therefore, we have induced mouse bone marrow mesenchymal stem cells (BMMSCs) into melanocytes (miMels) in the previous experiment. This experiment continues the previous experiment to further study the biological functions of miMels and their application in tissue engineering.
We examined whether miMels can produce active tyrosinase, melanin, and response to α-MSH. The ability of miMels to produce melanin to keratinocytes was tested by co-culture. By applying miMels to tissue-engineered skin, the survival and function of miMels on the surface of nude mice were verified.
MiMels can produce active tyrosinase and melanin, and can pass melanin to the co-cultured keratinocytes. Under the stimulation of α-MSH, the active tyrosinase and melanin content of miMels increased. We tried to apply it to the establishment of tissue-engineered skin and obtained tissue-engineered skin containing miMels. Then we tried to transplant tissue-engineered skin on the back skin of nude mice and succeeded. The transplanted miMels survived in local tissues, synthesized active tyrosinase and melanin, and expressed the marker protein of melanocytes.
In short, miMels can be used as a cell source for tissue engineering skin. MiMels not only have a typical melanocyte morphology but also have the same biological functions as normal melanocytes. What's more important is its successful application in mouse tissue-engineered experiments.
白癜风等色素脱失性疾病的手术治疗需要大量自体黑素细胞。本实验旨在探索间充质干细胞诱导的黑素细胞在临床治疗中的应用。因此，我们在之前的实验中已将小鼠骨髓间充质干细胞（BMMSCs）诱导为黑素细胞（miMels）。本实验延续之前的实验，进一步研究miMels的生物学功能及其在组织工程中的应用。
我们检测了miMels是否能产生活性酪氨酸酶、黑色素以及对α-MSH的反应。通过共培养测试miMels向角质形成细胞产生黑色素的能力。将miMels应用于组织工程皮肤，验证其在裸鼠体表的存活及功能。
miMels能产生活性酪氨酸酶和黑色素，并能将黑色素传递给共培养的角质形成细胞。在α-MSH刺激下，miMels的活性酪氨酸酶和黑色素含量增加。我们尝试将其应用于组织工程皮肤的构建，获得了含有miMels的组织工程皮肤。然后我们尝试将组织工程皮肤移植到裸鼠背部皮肤并取得成功。移植的miMels在局部组织中存活，合成活性酪氨酸酶和黑色素，并表达黑素细胞的标志物蛋白。
简而言之，miMels可作为组织工程皮肤的细胞来源。miMels不仅具有典型的黑素细胞形态，还具有与正常黑素细胞相同的生物学功能。更重要的是，它在小鼠组织工程实验中成功应用。
