Mangi Fayaz Hussain, Shaikh Tanweer Ahmed, Soria Daniele, Waryah Ali Muhammad, Ujjan Ikram Din, Qureshi Jawaid Naeem, Syed Binafsha Manzoor
Department of Clinical Research, Medical Research Centre, Liaquat University of Medical & Health Sciences, Jamshoro, Pakistan.
Department of Pathology, Liaquat University of Medical & Health Sciences, Jamshoro, Pakistan.
Saudi J Biol Sci. 2022 May;29(5):3929-3936. doi: 10.1016/j.sjbs.2022.03.014. Epub 2022 Mar 10.
Colorectal cancer (CRC) is one of the most common cancers worldwide. This study was designed to evaluate biological patterns, explore molecular classification and correlate with survival outcome in treatment naïve CRC patients.
Over 11 years consecutive series of 435 CRC patients were operated on as primary surgical therapy. A total of 201 CRC patients were included, whose complete set of clinical information was available, and their good quality tumour blocks were retrieved. Immunohistochemistry was used for tumour analysis, and partitional clustering was performed using R software for cluster analysis.
The median age was 43 (range 10-85) years; adenocarcinoma was the most commonly seen histological type. The great majority had positive CK20, CEA, E-Cadherin, Ki67, CDX2, and p53 expression. There were four distinct molecular classes found, whereas Ki67, CDX2, and p53 play the main role in partitioning. Younger age negatively impacted survival; overall and disease-specific survival was 26 months only with 50 months' longest survival.
Colorectal cancer is a biologically heterogeneous disease with at least four distinct molecular patterns, where cell proliferation and gene repair mechanisms appear to play the key role.
结直肠癌（CRC）是全球最常见的癌症之一。本研究旨在评估初治CRC患者的生物学模式，探索分子分类并将其与生存结果相关联。
连续11年对435例CRC患者进行了作为主要外科治疗的手术。共纳入201例CRC患者，其完整的临床信息可用，并获取了质量良好的肿瘤组织块。采用免疫组织化学进行肿瘤分析，并使用R软件进行分区聚类以进行聚类分析。
中位年龄为43岁（范围10 - 85岁）；腺癌是最常见的组织学类型。绝大多数患者的CK20、CEA、E-钙黏蛋白、Ki67、CDX2和p53表达呈阳性。发现了四种不同的分子类别，而Ki67、CDX2和p53在分区中起主要作用。年轻对生存有负面影响；总生存期和疾病特异性生存期仅为26个月，最长生存期为50个月。
结直肠癌是一种具有至少四种不同分子模式的生物学异质性疾病，其中细胞增殖和基因修复机制似乎起关键作用。
