Department of Gastroenterology, Hubei Third People's Hospital Affiliated to Jianghan University, Wuhan, Hubei 430030, China.
Emergency Department, Hubei Third People's Hospital Affiliated to Jianghan University, Wuhan, Hubei 430030, China.
Comput Math Methods Med. 2022 Jul 8;2022:8801484. doi: 10.1155/2022/8801484. eCollection 2022.
The lack of certain trace elements such as selenium, molybdenum, magnesium or related nutrients in the soil, water quality and food in the disease area, which caused disturbance of myocardium metabolism and resulted in injury and necrosis. The aim of the study was to explore the mechanism of ibuprofen alleviating myocardial injury caused by acute pancreatitis (AP).
We have established AP cell model and rat model. HE staining is used for histological examination. ELISA is used to determine the levels of proinflammatory cytokines (TNF- and IL-6) and markers of myocardial injury (LDH and CK-MB). qRT-PCR and Western blot are used to analyze the mRNA and protein levels of related genes.
The expression level of AIM2 was significantly increased in AP cells; downregulation of AIM2 alleviated inflammation and myocardial injury induced by AP cells; ibuprofen could inhibit the expression of AIM2 and alleviate inflammation and myocardial injury induced by AP cells. In vivo experiments have found that ibuprofen can inhibit the expression of AIM2 to alleviate myocardial injury in AP rat.
Ibuprofen can alleviate myocardial injury caused by acute pancreatitis by inhibiting the expression of AIM2.
疾病地区土壤、水质和食物中缺乏某些微量元素（如硒、钼、镁或相关营养素），导致心肌代谢紊乱，引起损伤和坏死。本研究旨在探讨布洛芬缓解急性胰腺炎（AP）引起的心肌损伤的机制。
建立 AP 细胞模型和大鼠模型。HE 染色进行组织学检查。ELISA 法测定促炎细胞因子（TNF-α和 IL-6）和心肌损伤标志物（LDH 和 CK-MB）水平。qRT-PCR 和 Western blot 分析相关基因的 mRNA 和蛋白水平。
AP 细胞中 AIM2 的表达水平明显升高；下调 AIM2 可减轻 AP 细胞引起的炎症和心肌损伤；布洛芬可抑制 AIM2 的表达，减轻 AP 细胞引起的炎症和心肌损伤。体内实验发现，布洛芬可通过抑制 AIM2 的表达来减轻 AP 大鼠的心肌损伤。
布洛芬通过抑制 AIM2 的表达缓解急性胰腺炎引起的心肌损伤。
