Department of Basic Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, China.
Department of Internal Medicine of Traditional Chinese Medicine (TCM), Nantong Hospital, Nantong, China.
Front Immunol. 2022 Jun 30;13:888918. doi: 10.3389/fimmu.2022.888918. eCollection 2022.
To explore the immune change of lung injury of Ulcerative colitis (UC) by observing the changes of inherent immunity and adaptive immunity of the lung and bowel in UC rat models after the treatment of Sodium Houttuyfonate combined with Matrine.
UC rat models were established with the mucous membrane of colon allergize combined with TNBS-alcohol enteroclysis for 1 week and 5 weeks. 1-week experimental rats were divided into normal group and model group, 5/each group. 5-weeks experimental rats were divided into normal group, model group, Sodium Houttuyfonate (2.9mg/ml) combined with Matrine (1.47mg/ml), and positive control sulfasalazine (10mg/ml), 5/each group. All rats were administered by gavage for 5 weeks. The histopathological and fibrotic changes in the lung and bowel were observed, and the expressions of Tumor Necrosis Factor (TNF)- α, interleukin (IL)-8 in the lung, bowel, and serum were detected by radio-immunity and immunohistochemistry, and the mRNA expressions of Toll-like receptor (TLR)-4, nuclear factor kappa (NF-κB), Macrophage migration inhibitory factor (MIF), Mucosal addressing cell adhesion molecule-1 (MadCAM1) and Pulmonary surfactant protein-A (SP-A) in the lung and bowel were detected by Real time-PCR.
Compared with the normal group, the model rats had significant histopathological and fibrotic changes both in the lung and bowel, and all treatment groups were improved. After treatment, TLR4, IL-8, MIF, and TNF-α in the lung decreased (P<0.05); NF-KB, IL-8, and MIF in the bowel increased (P<0.05); MadCAM1 both in lung and bowel decreased (P<0.05); SP-A decreased in bowel and increased in the lung (P<0.05).
The cause of lung injury in this model was found to be related to inherent immunity and adaptive immunity, while the cause of bowel injury in this model was found to be mainly related to adaptive immunity. Sodium Houttuyfonate combined with Matrine could improve bowel and lung injury.
通过观察溃疡性结肠炎（UC）大鼠模型肺和肠固有免疫和适应性免疫的变化，探讨苦参碱钠联合苦参碱对溃疡性结肠炎肺损伤的免疫调节作用。
采用结肠黏膜致敏联合 TNBS-乙醇灌肠法建立 UC 大鼠模型，1 周和 5 周后进行实验。1 周实验组大鼠分为正常组和模型组，每组 5 只；5 周实验组大鼠分为正常组、模型组、苦参碱钠（2.9mg/ml）联合苦参碱（1.47mg/ml）、阳性对照柳氮磺胺吡啶（10mg/ml），每组 5 只。所有大鼠均灌胃给药 5 周。观察肺和肠的组织病理学和纤维化变化，用放射免疫法和免疫组化法检测肺、肠、血清中肿瘤坏死因子（TNF）-α、白细胞介素（IL）-8 的表达，用 Real-time-PCR 检测肺和肠中 Toll 样受体（TLR）-4、核因子 kappa（NF-κB）、巨噬细胞移动抑制因子（MIF）、黏膜地址素细胞黏附分子-1（MadCAM1）和肺表面活性蛋白-A（SP-A）的 mRNA 表达。
与正常组相比，模型组大鼠肺和肠均有明显的组织病理学和纤维化改变，各治疗组均有改善。治疗后，肺组织 TLR4、IL-8、MIF 和 TNF-α降低（P<0.05）；肠组织 NF-κB、IL-8 和 MIF 增加（P<0.05）；肺和肠组织 MadCAM1 降低（P<0.05）；肠组织 SP-A 降低，肺组织 SP-A 增加（P<0.05）。
该模型肺损伤的发生与固有免疫和适应性免疫有关，而肠损伤的发生主要与适应性免疫有关。苦参碱钠联合苦参碱可改善肠和肺损伤。
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