Sarnatskaya Veronika, Shlapa Yuliia, Lykhova Alexandra, Brieieva Olga, Prokopenko Igor, Sidorenko Alexey, Solopan Serhii, Kolesnik Denis, Belous Anatolii, Nikolaev Vladimir
Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Ukraine.
V. I. Vernadsky Institute of General and Inorganic Chemistry of the NAS of Ukraine, 32/34, Palladina Ave., Kyiv, 03142, Ukraine.
Heliyon. 2022 Mar 24;8(3):e09163. doi: 10.1016/j.heliyon.2022.e09163. eCollection 2022 Mar.
Over the recent years, carbon particles have gained relevance in the field of biomedical application to diminish the level of endo-/exogenous intoxication and oxidative stress products, which occur at different pathological states. However, it is very important that such carbon particles, specially developed for parenteral administration or usage, possess a high adsorption potential and can remove hazard toxic substances of the hydrophilic, hydrophobic and amphiphilic nature usually accumulated in the blood due to the disease, and be absolutely safe for normal living cells and tissues of organism. In this work, the stable monodisperse suspension containing very small-sized (D = 1125.3 ± 243.8 nm) and highly pure carbon particles with an excellent accepting ability were obtained. UV-spectra, fluorescence quenching constant and binding association constant were provided by the information about conformational alterations in an albumin molecule in presence of carbon particles, about the dynamic type of quenching process and low binding affinity between carbon and protein. The later was confirmed by DSC method. cell culture experiments showed that carbon particles did not possess any cytotoxic effect towards all testing the normal cell lines of different histogenesis, did not show genotoxic effects and were absolutely safe for experimental animals during and after their parenteral administration. These observations may provide more information about how to develop a safe preparation of carbon particles for different biomedical applications, in particular, as a mean for intracorporeal therapy of various heavy diseases accompanied by the increased endogenous intoxication and the level of oxidative stress.
近年来，碳颗粒在生物医学应用领域变得越发重要，其可降低在不同病理状态下出现的内源性/外源性中毒水平以及氧化应激产物。然而，对于专门为肠胃外给药或使用而研发的此类碳颗粒而言，非常重要的一点是，它们需具备高吸附潜力，能够清除通常因疾病而在血液中积累的具有亲水性、疏水性及两亲性的有害有毒物质，并且对生物体的正常活细胞和组织绝对安全。在这项工作中，获得了一种稳定的单分散悬浮液，其中含有尺寸非常小（D = 1125.3 ± 243.8 nm）且纯度高、具有出色接受能力的碳颗粒。通过碳颗粒存在时白蛋白分子构象改变的信息、猝灭过程的动态类型以及碳与蛋白质之间低结合亲和力的信息，得到了紫外光谱、荧光猝灭常数和结合缔合常数。后者通过差示扫描量热法得到证实。细胞培养实验表明，碳颗粒对所有测试的不同组织发生的正常细胞系均无任何细胞毒性作用，未显示出遗传毒性作用，并且在肠胃外给药期间及之后对实验动物绝对安全。这些观察结果可能会为如何开发用于不同生物医学应用的安全碳颗粒制剂提供更多信息，特别是作为治疗伴有内源性中毒增加和氧化应激水平升高的各种重症的体内治疗手段。
