Gerashchenko Bogdan I, Sydorenko Oleksii S, Snezhkova Elisaveta A, Klymchuk Dmytro O, Nikolaev Vladimir G
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Vasylkivska Str. 45, Kyiv 03022, Ukraine.
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Vasylkivska Str. 45, Kyiv 03022, Ukraine.
Micron. 2018 Mar;106:42-47. doi: 10.1016/j.micron.2018.01.001. Epub 2018 Jan 4.
In extracorporeal blood purification, such as hemoperfusion, activated carbon (activated charcoal) beads are commonly used as an adsorbent, but their judgment in terms of extent of microparticle release is of great importance since the microparticles may represent the risk of entering the bloodstream. To quantitatively assess the release of carbon microparticles (CMPs) in the samples of the aqueous perfusion medium, in which the beads have been perfused, the calibration procedure with different concentrations of CMPs is likely to be needed. For this purpose, carbon beads were mechanically crushed to a fine powder, whose microparticles (<10 μm) were then serially diluted in the aqueous medium within the wide range of concentrations (0.2-100 μg/ml). To test these concentrations of CMPs, the micro-aliquots of each dilution of suspended CMPs were dried on a surface of hydrophobic membrane and at the optical magnification of 20× the dry residues were than analyzed by measuring the sum of densities. This simple and affordable technique was shown to be considerably more sensitive than spectrophotometry of the aqueous suspensions of CMPs.
在体外血液净化中，如血液灌流，活性炭珠通常用作吸附剂，但由于微粒可能存在进入血液的风险，因此对其微粒释放程度的判断至关重要。为了定量评估在已灌注珠子的水性灌注介质样品中碳微粒（CMPs）的释放情况，可能需要采用不同浓度CMPs的校准程序。为此，将碳珠机械粉碎成细粉，然后将其微粒（<10μm）在水性介质中进行一系列浓度范围（0.2 - 100μg/ml）的稀释。为了测试这些CMPs浓度，将悬浮CMPs每次稀释的微量样品干燥在疏水膜表面，然后在20倍光学放大倍数下通过测量密度总和来分析干燥残留物。结果表明，这种简单且经济实惠的技术比CMPs水性悬浮液的分光光度法灵敏得多。
