Whitaker Eugene J, Darcey Caitlin D, Somerset Susan M
Department of Restorative Dentistry, Kornberg School of Dentistry, Temple University, Philadelphia, Pennsylvania, USA.
Department of Periodontology, Naval Postgraduate Dental School, Bethesda, Maryland, USA.
Contemp Clin Dent. 2022 Apr-Jun;13(2):135-139. doi: 10.4103/ccd.ccd_656_20. Epub 2022 Jun 21.
Periodontitis is a persistent infection of the tissues surrounding the teeth characterized by inflamed microvasculature, and is associated with increased systemic platelet activation.
The purpose of this study was to assess the platelet aggregating potential of the red-complex bacterium . A second-related objective was to ascertain the effect of dual platelet inhibitors on -platelet interaction.
These experiments were done in a basic science laboratory combining isolated human platelets with isolated bacterial cells.
Dilutions of cells were counted by quantitative polymerase chain reaction. Aggregation was assayed in a platelet aggregometer after adding cells or sonic extracts to gel filtered platelets, some of which were preincubated with the dual platelet inhibitors aspirin plus clopidogrel.
Platelets aggregate when exposed to cells or sonic extracts and dilution results in increased lag times and decreased aggregation. Platelets preincubated with the combination of aspirin plus clopidogrel do not aggregate in response to .
Within the limitations of this study, cells aggregate human platelets and the activity can be attenuated by diluting the cells and blocked by the combination of aspirin plus clopidogrel.
牙周炎是牙齿周围组织的持续性感染，其特征为微血管发炎，并与全身血小板活化增加有关。
本研究的目的是评估红色复合体细菌的血小板聚集潜能。第二个相关目标是确定双重血小板抑制剂对血小板相互作用的影响。
这些实验在基础科学实验室进行，将分离出的人类血小板与分离出的细菌细胞相结合。
通过定量聚合酶链反应对细胞稀释液进行计数。在向凝胶过滤血小板中添加细胞或超声提取物后，使用血小板聚集仪测定聚集情况，其中一些血小板预先与双重血小板抑制剂阿司匹林加氯吡格雷孵育。
血小板暴露于细胞或超声提取物时会聚集，稀释会导致延迟时间增加和聚集减少。预先用阿司匹林加氯吡格雷组合孵育的血小板对[具体物质]无反应而不聚集。
在本研究的局限性内，[具体细菌]细胞可使人类血小板聚集，通过稀释细胞可减弱该活性，阿司匹林加氯吡格雷组合可阻断该活性。
