Tropical Aquaculture Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya, China.
Sanya Tropical Fisheries Research Institute, Sanya, China.
Front Cell Infect Microbiol. 2022 Jun 30;12:945152. doi: 10.3389/fcimb.2022.945152. eCollection 2022.
In the study, the parasite from the yellowfin tuna () was separated, and morphological observation and molecular identification were carried out. Our results showed that the parasite was similar to sp. Its cephalothorax was covered by spherical to spherical non-branched nipples of almost the same size, which were very similar in shape and arrangement. A pair of slightly larger, the unbranched antenna was present on the outer margin of the small papillae-covered area. The gene sequence of COX1 with a length of 1,558 bp in the mitochondria of the parasite was 100% similar to sp. (MZ934363). The mitochondrial genome had a total length of 14,620 bp. It consisted of 36 genes (12 protein-coding, 22 transfer RNAs and 2 ribosomal RNAs) and a dummy control region, but the mitochondrial genome had no ATP8 gene. Morphological observation showed that sp. was dark red, with a convex cephalothorax, with a total length of 8.42 cm, parasitic on the dorsal side of yellowfin tuna. sp. included the cephalothorax, neck, trunk, abdomen and egg belt. This study was the first report on the mitochondrial genome of sp. The results provide basic data for further identifying the parasites of genus.
在这项研究中，分离了黄鳍金枪鱼（）中的寄生虫，并进行了形态观察和分子鉴定。我们的结果表明，该寄生虫与 sp. 相似。它的头胸甲被几乎相同大小的球形至球形无分枝乳突覆盖，形状和排列非常相似。一对稍大的无分枝触角位于小乳突覆盖区域的外缘。寄生虫线粒体 COX1 基因长 1558bp，与 sp. (MZ934363) 的相似度为 100%。线粒体基因组全长 14620bp，由 36 个基因（12 个蛋白编码、22 个转移 RNA 和 2 个核糖体 RNA）和一个假控制区组成，但线粒体基因组没有 ATP8 基因。形态观察表明，sp. 呈暗红色，头胸甲凸起，全长 8.42cm，寄生在黄鳍金枪鱼的背部。sp. 包括头胸甲、颈、躯干、腹部和卵带。本研究首次报道了 sp. 的线粒体基因组。研究结果为进一步鉴定属寄生虫提供了基础数据。
