Dąbkowski Krzysztof, Łabędź-Masłowska Anna, Dołęgowska Barbara, Safranow Krzysztof, Budkowska Marta, Zuba-Surma Ewa, Starzyńska Teresa
Department of Gastroenterology, Faculty of Medicine and Dentistry, Pomeranian Medical University, Unii Lubelskiej 1, 71-252 Szczecin, Poland.
Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Krakow, Poland.
Stem Cells Int. 2022 Jul 8;2022:5395248. doi: 10.1155/2022/5395248. eCollection 2022.
A growing number of studies indicate the potential involvement of various populations of bone marrow-derived stem cells (BMSCs) in tissue repair. However, the mobilization of BMSCs to the peripheral blood (PB) in acute and chronic pancreatitis (AP and CP) has not been investigated. A total of 78 patients were assigned into AP, CP, and healthy control groups in this study. Using flow cytometry, we found that VSELs, EPCs, and CD133SCs were mobilized to the PB of patients with both AP and CP. Interestingly, AP and CP patients exhibited lower absolute number of circulating MSCs in the PB compared to healthy individuals. SC mobilization to the PB was more evident in patients with AP than CP and in patients with moderate/severe AP than mild AP. Using ELISA, we found a significantly increased HGF concentration in the PB of patients with AP and SDF1 in the PB of patients with CP. We noted a significant positive correlation between SDF1 concentration and the mobilized population of CD133SCs in AP and between C5a and the mobilized population of VSELs moderate/severe AP. Thus, bone marrow-derived SCs may play a role in the regeneration of pancreatic tissue in both AP and CP, and mobilization of VSELs to the PB depends on the severity of AP.
越来越多的研究表明，各种骨髓源性干细胞（BMSC）群体可能参与组织修复。然而，急性和慢性胰腺炎（AP和CP）中BMSC向外周血（PB）的动员情况尚未得到研究。本研究共将78例患者分为AP组、CP组和健康对照组。通过流式细胞术，我们发现VSEL、EPC和CD133SC均被动员至AP和CP患者的PB中。有趣的是，与健康个体相比，AP和CP患者PB中循环MSC的绝对数量较低。SC向PB的动员在AP患者中比CP患者更明显，在中度/重度AP患者中比轻度AP患者更明显。通过ELISA，我们发现AP患者PB中的HGF浓度显著升高，CP患者PB中的SDF1浓度显著升高。我们注意到，在AP中，SDF1浓度与CD133SC的动员群体之间存在显著正相关，在中度/重度AP中，C5a与VSEL的动员群体之间存在显著正相关。因此，骨髓源性SC可能在AP和CP的胰腺组织再生中发挥作用，VSEL向PB的动员取决于AP的严重程度。
