Zhang Deyin, Zhang Xiaoxue, Li Fadi, Zhao Yuan, Li Xiaolong, Wang Jianghui, Zhao Liming, Yang Xiaobin, Zhang Yukun, Xu Dan, Cheng Jiangbo, Li Wenxin, Lin Changchun, Zhou Bubo, Wang Weimin
The State Key Laboratory of Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Engineering Research Center of Grassland Industry, Ministry of Education, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
Front Vet Sci. 2022 Jun 30;9:925928. doi: 10.3389/fvets.2022.925928. eCollection 2022.
Hematological traits are important indexes to evaluate health status and immunological conditions in human and livestock. In this study, we measured the hematologic indexes of 819 male Hu lambs and performed the descriptive statistical analysis. The results showed the coefficients of variation of partial indexes were >10%, and the heritability for mean erythrocyte volume (MCV), white blood cell count (WBC), hemoglobin concentration (HGB), hematocrit (HCT), and red blood cell (RBC) distribution-standard deviation (RDW_SD) were medium to high, ranging from 0.17 to 0.43. In addition, Interleukin 18 (), as an important regulator of both innate and acquired immune responses, was selected as candidate gene and subjected to the expression profile analysis, single nucleotide polymorphism (SNP) scanning and association analysis by using quantitative real-time PCR (qRT-PCR), PCR amplification, Sanger sequencing, and KASP genotyping. The results of qRT-PCR indicated that is predominantly expressed in lymph and lung compared with that in the other tested tissues. In addition, three novel polymorphisms (g. 24991544 A > G, g. 24991651 A > G, and g. 24991749 C > T) were identified in , and the three SNPs were in a strong linkage state. Therefore, only a SNP was genotyped and performed association analysis in the enlarged experimental population, the result of association analysis demonstrated that the polymorphism g. 24991651 A > G was significantly associated with RBC, MCV, MCHC, and RDW_CV. These results will provide the reference values and the novel genetic markers of hematological parameters in sheep.
血液学特征是评估人类和家畜健康状况及免疫状态的重要指标。在本研究中，我们测定了819只雄性湖羊羔羊的血液学指标，并进行了描述性统计分析。结果显示部分指标的变异系数>10%，平均红细胞体积（MCV）、白细胞计数（WBC）、血红蛋白浓度（HGB）、血细胞比容（HCT）和红细胞（RBC）分布标准差（RDW_SD）的遗传力为中到高，范围从0.17到0.43。此外，白细胞介素18（）作为先天性和获得性免疫反应的重要调节因子，被选为候选基因，并通过定量实时PCR（qRT-PCR）、PCR扩增、桑格测序和KASP基因分型进行表达谱分析、单核苷酸多态性（SNP）扫描和关联分析。qRT-PCR结果表明，与其他测试组织相比，在淋巴和肺中表达量最高。此外，在中鉴定出三个新的多态性（g.24991544 A>G、g.24991651 A>G和g.24991749 C>T），这三个SNP处于强连锁状态。因此，仅对一个SNP在扩大的实验群体中进行基因分型和关联分析，关联分析结果表明多态性g.24991651 A>G与RBC、MCV、MCHC和RDW_CV显著相关。这些结果将为绵羊血液学参数提供参考值和新的遗传标记。
