Department of Developmental and Cell Biology, University of California, Irvine, California, USA.
Center for Complex Biological Systems, University of California, Irvine, California, USA.
Dev Growth Differ. 2022 Aug;64(6):297-305. doi: 10.1111/dgd.12799. Epub 2022 Aug 4.
Early embryonic cell fates are specified through coordinated integration of transcription factor activities and epigenetic states of the genome. Foxh1 is a key maternal transcription factor controlling the mesendodermal gene regulatory program. Proteomic interactome analyses using FOXH1 as a bait in mouse embryonic stem cells revealed that FOXH1 interacts with PRC2 subunits and HDAC1. Foxh1 physically interacts with Hdac1, and confers transcriptional repression of mesendodermal genes in Xenopus ectoderm. Our findings reveal a central role of Foxh1 in coordinating the chromatin states of the Xenopus embryonic genome.
早期胚胎细胞的命运是通过转录因子活性和基因组的表观遗传状态的协调整合来确定的。Foxh1 是一种关键的母源转录因子，控制中胚层和内胚层的基因调控程序。使用 FOXH1 作为诱饵的蛋白质组相互作用分析在小鼠胚胎干细胞中表明，FOXH1 与 PRC2 亚基和 HDAC1 相互作用。Foxh1 与 Hdac1 相互作用，并赋予 Xenopus 外胚层中中胚层和内胚层基因的转录抑制。我们的发现揭示了 Foxh1 在协调 Xenopus 胚胎基因组染色质状态中的核心作用。
