化癥回生片治疗四氯化碳致肝纤维化的药物反应生物标志物。
Drug response biomarkers of Pien Tze Huang treatment for hepatic fibrosis induced by carbon tetrachloride.
机构信息
School of Life Sciences, Anhui Medical University, Hefei 230032, China.
2 Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Ministry of Education, Shanghai Jiao Tong University, Shanghai 200030, China.
出版信息
J Tradit Chin Med. 2022 Aug;42(4):530-538. doi: 10.19852/j.cnki.jtcm.2022.04.003.
OBJECTIVE
To explore biomarkers of Pien Tze Huang that ameliorated the symptoms of hepatic fibrosis.
METHODS
Two groups of carbon tetrachloride-induced hepatic fibrosis (HF) mice model were constructed in our study: one group received PZH treatment and another group received no treatment. We performed this study to investigate the role of PZH in the regulation process of hepatic fibrosis.
RESULTS
We identified 31 down-regulated and 39 up-regulated miRNAs using small RNA-seq analysis. Combining RNA-Seq data analysis, our study revealed 7 significant target genes (Sp4, Slc2a6, Tln2, Hmga2, Ank3, Pax9, Fgf9). The results of real-time quantitative polymerase chain reaction analysis suggested that the expression level of 6 genes (Sp4, Tln2, Hmga2, Ank3, Pax9, Fgf9) were down-regulated compared to control group. On the other hand, the expression level of Slc2a6 appeared to be up-regulated. The protein mass spectrometry showed that PZH group had lower protein expression of Tln2 compared to control group.
CONCLUSION
We identified 7 genes that were significantly related to PZH response in HF mice using multiple conjoint analysis methods. These genes could participate in underlying regulation mechanism of hepatic fibrosis during PZH treatment.
目的
探索对肝纤维化症状有改善作用的片仔癀生物标志物。
方法
本研究构建了两组四氯化碳诱导的肝纤维化（HF）小鼠模型：一组接受片仔癀治疗，另一组未接受治疗。我们进行这项研究旨在探讨片仔癀在肝纤维化调控过程中的作用。
结果
我们通过小 RNA-seq 分析鉴定出 31 个下调和 39 个上调的 miRNA。结合 RNA-Seq 数据分析，本研究揭示了 7 个显著的靶基因（Sp4、Slc2a6、Tln2、Hmga2、Ank3、Pax9、Fgf9）。实时定量聚合酶链反应分析结果表明，与对照组相比，6 个基因（Sp4、Tln2、Hmga2、Ank3、Pax9、Fgf9）的表达水平下调。另一方面，Slc2a6 的表达水平似乎上调。蛋白质质谱分析显示，与对照组相比，片仔癀组 Tln2 的蛋白表达水平较低。
结论
我们使用多种联合分析方法鉴定出 7 个与 HF 小鼠片仔癀反应显著相关的基因。这些基因可能参与片仔癀治疗期间肝纤维化的潜在调控机制。
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