巴西马拉尼昂州蚊子（双翅目：蚊科）中沃尔巴克氏体的检测。

Detection of Wolbachia in Mosquitoes (Diptera: Culicidae) in the State of Maranhão, Brazil.

机构信息

Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal do Maranhão, Avenida dos Portugueses 1966, Campus do Bacanga, 65080-805, São Luís, Maranhão, Brazil.

Laboratório de Mosquitos Transmissores de Hematozoários, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Brasil, Av. Brasil, 4365, Manguinhos, 21040-900, Rio de Janeiro-RJ, Brazil.

出版信息

J Med Entomol. 2022 Sep 14;59(5):1831-1836. doi: 10.1093/jme/tjac092.

DOI:10.1093/jme/tjac092

PMID:35849008

Abstract

Recently, the endobacteria Wolbachia has emerged as a biological tool for the control of arboviruses. Thus, we investigated the rate of natural infection by Wolbachia in Culicidae species from Maranhão, Brazil. For this, we amplified the Wolbachia surface protein gene (wsp) from mosquitoes collected in six localities of Maranhão, and positive samples were subjected to new analysis using group-specific primers. In total, 448 specimens comprising 6 genera and 18 species of mosquitoes were analyzed. Wolbachia DNA was PCR-detected in 7 species, three of which are new records: Aedes scapularis (Rondani, 1848), Coquillettidia juxtamansonia (Chagas, 1907) and Cq. venezuelensis (Theobald, 1912), in addition to Ae. albopictus (Skuse, 1894) and Culex quinquefasciatus Say, 1823, which are commonly described as permissive to maintain this bacterium in natural environments, and two species of the subgenera Anopheles (Nyssorhynchus) Blanchard, 1902 and Culex (Melanoconion) Theobald, 1903 which could not be identified at species level. The infection rate of all species ranged from 0 to 80%, and the average value was 16.5%. This study increases the knowledge about the prevalence of Wolbachia in the culicid fauna and may help in selecting strains for biological control purposes.

摘要

最近，内共生菌沃尔巴克氏体（Wolbachia）作为控制虫媒病毒的生物工具而受到关注。因此，我们调查了巴西马拉尼昂州库蚊科物种中沃尔巴克氏体的自然感染率。为此，我们从马拉尼昂州的六个地点采集的蚊子中扩增了沃尔巴克氏体表面蛋白基因（wsp），并对阳性样本使用组特异性引物进行了新的分析。总共分析了 448 只包含 6 属和 18 种蚊子的标本。在 7 种蚊子中检测到了沃尔巴克氏体 DNA，其中 3 种是新记录：刺扰伊蚊（Aedes scapularis）（Rondani，1848）、库蚊东方亚种（Coquillettidia juxtamansonia）（Chagas，1907）和 Cq. venezuelensis（Theobald，1912），此外还有白纹伊蚊（Aedes albopictus）（Skuse，1894）和三带喙库蚊（Culex quinquefasciatus Say，1823），这两种蚊子通常被描述为在自然环境中允许维持这种细菌，还有两种属于按蚊亚属（Nyssorhynchus）和库蚊属（Melanoconion）的蚊子未能鉴定到种。所有蚊子的感染率从 0 到 80%不等，平均值为 16.5%。本研究增加了对沃尔巴克氏体在库蚊区系中流行程度的了解，这可能有助于选择用于生物控制目的的菌株。