Department of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China.
Department of Clinical Medicine, The Second Clinical School of Guangzhou Medical University, Guangzhou, China.
Lab Med. 2023 Jan 5;54(1):56-64. doi: 10.1093/labmed/lmac030.
To evaluate the accuracy of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in community or primary-care settings.
We systematically searched the Web of Science, Embase, PubMed, and Cochrane Library databases. We conducted quality evaluation using ReviewManager software (version 5.0). We then used MetaDisc software (version 1.4) and Stata software (version 12.0) to build forest plots, along with a Deeks funnel plot and a bivariate boxplot for analysis.
Overall, the sensitivity, specificity, and diagnostic odds ratio were 0.79, 0.97, and 328.18, respectively. The sensitivity for the subgroup with RNA extraction appeared to be higher, at 0.88 (0.86-0.90), compared to the subgroup without RNA extraction, at 0.50 (0.45-0.55), with no significant difference in specificity.
RT-LAMP assay exhibited high specificity regarding current SARS-CoV-2 infection. However, its overall sensitivity was relatively moderate. Extracting RNA was found to be beneficial in improving sensitivity.
评估逆转录环介导等温扩增（RT-LAMP）检测在社区或初级保健环境中快速检测严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的准确性。
我们系统地检索了 Web of Science、Embase、PubMed 和 Cochrane Library 数据库。我们使用 ReviewManager 软件（版本 5.0）进行质量评估。然后，我们使用 MetaDisc 软件（版本 1.4）和 Stata 软件（版本 12.0）构建森林图，并进行 Deeks 漏斗图和双变量箱线图分析。
总体而言，敏感性、特异性和诊断优势比分别为 0.79、0.97 和 328.18。对于进行 RNA 提取的亚组，敏感性似乎更高，为 0.88（0.86-0.90），而未进行 RNA 提取的亚组为 0.50（0.45-0.55），特异性无显著差异。
RT-LAMP 检测法在当前 SARS-CoV-2 感染方面具有较高的特异性。然而，其整体敏感性相对适中。提取 RNA 有助于提高敏感性。
