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一种用于直接可视化检测 SARS-CoV-2 的敏感逆转录环介导等温扩增检测方法。

A Sensitive Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Direct Visual Detection of SARS-CoV-2.

机构信息

1Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

2Department of Pathology, Hospital Sungai Buloh, Selangor, Malaysia.

出版信息

Am J Trop Med Hyg. 2020 Dec;103(6):2350-2352. doi: 10.4269/ajtmh.20-1079. Epub 2020 Oct 23.

DOI:10.4269/ajtmh.20-1079
PMID:33098286
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7695063/
Abstract

A simple and rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of SARS-CoV-2. The RT-LAMP assay was highly specific for SARS-CoV-2 and was able to detect one copy of transcribed SARS-CoV-2 RNA within 24 minutes. Assay validation performed using 50 positive and 32 negative clinical samples showed 100% sensitivity and specificity. The RT-LAMP would be valuable for clinical diagnosis and epidemiological surveillance of SARS-CoV-2 infection in resource-limited areas as it does not require the use of sophisticated and costly equipment.

摘要

一种简单快速的逆转录环介导等温扩增（RT-LAMP）检测方法被开发出来用于检测 SARS-CoV-2。该 RT-LAMP 检测方法对 SARS-CoV-2 具有高度特异性，能够在 24 分钟内检测到一个转录的 SARS-CoV-2 RNA 拷贝。使用 50 份阳性和 32 份阴性临床样本进行的检测验证表明，该检测方法具有 100%的灵敏度和特异性。该 RT-LAMP 不需要使用复杂和昂贵的设备，因此对于资源有限地区的 SARS-CoV-2 感染的临床诊断和流行病学监测具有重要价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4a/7695063/d66f7404899f/tpmd201079f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4a/7695063/d66f7404899f/tpmd201079f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/db4a/7695063/d66f7404899f/tpmd201079f1.jpg

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Development of a Novel Reverse Transcription Loop-Mediated Isothermal Amplification Method for Rapid Detection of SARS-CoV-2.
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Diagnostics (Basel). 2022 Mar 29;12(4):848. doi: 10.3390/diagnostics12040848.
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