Cheng Yuan, Zheng Liangyu
Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, People's Republic of China.
Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, People's Republic of China.
Colloids Surf B Biointerfaces. 2022 Sep;217:112690. doi: 10.1016/j.colsurfb.2022.112690. Epub 2022 Jul 6.
Here the metal-organic framework material MIL-88A is used to purify and immobilize an acyltransferase from Mycobacterium smegmatis (MsAcT) simultaneously from the broken bacterial liquid. Regarding the possibility that the MsAcT@MIL-88A may display weak stability in its application, a silica layer is further introduced around it as a "shield" to protect the enzyme from degradation. The obtained MsAcT@MIL-88A@silica can exhibit high activity recovery, excellent thermal, pH, and storage stabilities compared with those of MsAcT@MIL-88A. The MsAcT@MIL-88A@silica can also be effectively recycled, and its initial activity of 84.0 % ± 1.2 % can be retained after the 5th cycle for N-acylation reaction in water. More importantly, the MsAcT@MIL-88A@silica can display much higher catalytic activity towards the reactions between ethyl or vinyl esters and aniline than those of free MsAcT and MsAcT@MIL-88A in aqueous media. This study provides a simple and inexpensive strategy to prepare MsAcT@MIL-88A@silica with high activity, stability, and excellent recyclability, and highlights its application potential as a biocatalyst.
在此，金属有机框架材料MIL-88A用于从破碎的菌液中同时纯化和固定耻垢分枝杆菌的酰基转移酶（MsAcT）。考虑到MsAcT@MIL-88A在其应用中可能表现出较弱的稳定性，在其周围进一步引入二氧化硅层作为“保护罩”，以保护酶不被降解。与MsAcT@MIL-88A相比，所获得的MsAcT@MIL-88A@二氧化硅可表现出高活性回收率、优异的热稳定性、pH稳定性和储存稳定性。MsAcT@MIL-88A@二氧化硅也可有效循环利用，在水相中进行N-酰化反应的第5次循环后，其初始活性可保留84.0%±1.2%。更重要的是，在水介质中，MsAcT@MIL-88A@二氧化硅对乙酯或乙烯基酯与苯胺之间反应的催化活性比游离MsAcT和MsAcT@MIL-88A高得多。本研究提供了一种简单且廉价的策略来制备具有高活性、稳定性和优异可循环性的MsAcT@MIL-88A@二氧化硅，并突出了其作为生物催化剂的应用潜力。
