发现糖基化萘甲霉素及其糖基化的阐明。
Discovery of glycosylated naphthacemycins and elucidation of the glycosylation.
机构信息
Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, No. 600 Changjiang Road, Xiangfang District, Harbin, 150030, PR China.
State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, University of Chinese Academy of Sciences, Chinese Academy of Sciences Chinese Academy of Sciences, 345 Lingling Road, Shanghai, 200032, PR China.
出版信息
Biochem Biophys Res Commun. 2022 Sep 24;622:122-128. doi: 10.1016/j.bbrc.2022.06.095. Epub 2022 Jul 9.
Two glycosylated naphthacemycins (naphthacemycins D and D) were identified in Streptomyces sp. N12W1565. These two compounds not only showed antimicrobial potential against bacteria but also exhibited more aqueous solubility than naphthacemycins. Furthermore, the whole genome of Streptomyces sp. N12W1565 has been sequenced, the natY gene, located outside the biosynthetic gene cluster encoding a D-glucose glycosyltransferase, was identified to mediate glycosylation in the phenolic hydroxyl of the naphthacemycin core scaffold. Glycosyltransferase was elucidated in vitro by using a homologous enzyme, which showed potential as a biocatalyst.
从链霉菌 N12W1565 中鉴定出两种糖基化萘甲霉素（萘甲霉素 D 和 D）。这两种化合物不仅对细菌具有抗菌潜力，而且比萘甲霉素具有更高的水溶性。此外，链霉菌 N12W1565 的全基因组已测序，位于生物合成基因簇外的 natY 基因被鉴定为介导萘甲霉素核心支架中酚羟基的糖基化。通过使用同源酶在体外阐明了糖基转移酶，该酶显示出作为生物催化剂的潜力。
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