Institute of Chemical Biology and Fundamental Medicine, Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Novosibirsk National Research State University, Novosibirsk, Russia.
Bull Exp Biol Med. 2022 Jul;173(3):384-389. doi: 10.1007/s10517-022-05554-8. Epub 2022 Jul 19.
Contamination of the sample with DNA is a problem when isolating RNA by phenol-chloroform extraction, and DNase treatment introduces an additional error in the analysis of gene expression. Bearing in mind the ability of LiCl to precipitate RNA selectively, we studied the possibility and advantage of using LiCl as a precipitation agent in the protocol for RNA precipitation [3] from frozen segments of large vessels. Combined use of LiCl with isopropanol as precipitating agents (optimal ratio 2.5 M and 40%, respectively) significantly eliminates negative effects of using this salt (inhibition of reverse transcription and low RNA yield).
用苯酚-氯仿抽提法分离 RNA 时，样本受到 DNA 污染是一个问题，而 DNase 处理会在基因表达分析中引入额外的误差。考虑到 LiCl 沉淀 RNA 的选择性，我们研究了在从大型血管冷冻切片中沉淀 RNA 的方案[3]中使用 LiCl 作为沉淀剂的可能性和优势。将 LiCl 与异丙醇联合使用作为沉淀剂（最佳比例分别为 2.5 M 和 40%）可显著消除使用该盐（抑制反转录和低 RNA 产量）的负面影响。
