Cevik Mehmet, Gobeka Hamidu Hamisi, Aydemir Orhan
Department of Ophthalmology, Agri Ibrahim Cecen University Faculty of Medicine, Agri, Turkey.
Department of Ophthalmology, Firat University Faculty of Medicine, Elazig, Turkey.
Int Ophthalmol. 2023 Jan;43(1):249-260. doi: 10.1007/s10792-022-02424-0. Epub 2022 Jul 19.
To investigate vascular endothelial growth factor (VEGF) and proliferating cell nuclear antigen (PCNA) immunoreactivities, as well as apoptosis and oxidative stress levels in Streptozotocin (STZ)-induced diabetic rats, and determine how neferine affected these parameters.
Thirty-five male Sprague Dawley rats were divided into five groups of seven. Fasting blood glucose was measured 72 h after diabetes mellitus (DM) induction in 21 rats using 60 mg/kg STZ dissolved in 0.4 ml (0.1 M) sodium-citrate buffer (pH:4.5), with values > 250 mg/dl considered diabetic. Group 1 received no treatment. Group 3 (healthy rats) received daily intraperitoneal (IP) 4 mg/kg neferine. Following DM induction: Group 2 (sham) received daily IP 0.25 ml/kg 0.9% normal saline; Group 4 received single IP 0.01 mL (2.5 mg/kg) bevacizumab, followed by daily IP 0.25 mL/kg 0.9% normal saline; and Group 5 received daily IP 4 mg/kg neferine. Total antioxidant capacity (TAC) and total oxidative stress (TOS) levels in serum and ocular tissue homogenates were evaluated using ELISA. TUNEL method was used for determining apoptosis and immuno-histochemical staining for PCNA and VEGF immunoreactivities.
Group 5 had significantly higher TAC and lower TOS in serum and ocular tissue homogenates than Group 4 (p < 0.05). Despite significantly lower VEGF levels and apoptosis (p < 0.05), there was no significant change in PCNA immunoreactivity in Group 5 (p > 0.05).
DM was associated with lower TAC, higher TOS and apoptotic cells, as well as VEGF and PCNA immunoreactivities in the retina. Neferine altered parameters other than PCNA in the opposite direction, demonstrating reductive effects on DM.
研究链脲佐菌素（STZ）诱导的糖尿病大鼠的血管内皮生长因子（VEGF）和增殖细胞核抗原（PCNA）免疫反应性，以及细胞凋亡和氧化应激水平，并确定甲基莲心碱如何影响这些参数。
将35只雄性Sprague Dawley大鼠分为5组，每组7只。使用溶解于0.4 ml（0.1 M）柠檬酸钠缓冲液（pH：4.5）中的60 mg/kg STZ对21只大鼠进行糖尿病（DM）诱导，72小时后测量空腹血糖，血糖值>250 mg/dl被视为糖尿病。第1组不接受治疗。第3组（健康大鼠）每天腹腔注射（IP）4 mg/kg甲基莲心碱。DM诱导后：第2组（假手术组）每天腹腔注射0.25 ml/kg 0.9%生理盐水；第4组单次腹腔注射0.01 mL（2.5 mg/kg）贝伐单抗，随后每天腹腔注射0.25 mL/kg 0.9%生理盐水；第5组每天腹腔注射4 mg/kg甲基莲心碱。使用ELISA评估血清和眼组织匀浆中的总抗氧化能力（TAC）和总氧化应激（TOS）水平。采用TUNEL法测定细胞凋亡，并对PCNA和VEGF免疫反应性进行免疫组织化学染色。
第5组血清和眼组织匀浆中的TAC显著高于第4组，TOS显著低于第4组（p<0.05）。尽管第5组的VEGF水平和细胞凋亡显著降低（p<0.05），但其PCNA免疫反应性无显著变化（p>0.05）。
DM与视网膜中较低的TAC、较高的TOS和凋亡细胞以及VEGF和PCNA免疫反应性相关。甲基莲心碱使除PCNA外的参数朝相反方向改变，表明其对DM具有减轻作用。
