采用双重免疫组织化学法评估 OCT3/4 与口腔鳞状细胞癌中 GLUT1 和 CD105 的相关性。

Assessment of the association of OCT3/4 with GLUT1 and CD105 in oral squamous cell carcinoma using dual immunohistochemistry.

机构信息

Department of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.

Cancer Preclinical Imaging Group, Preclinical Core Facility, Tehran University of Medical Sciences, Tehran, Iran.

出版信息

BMC Oral Health. 2022 Jul 19;22(1):300. doi: 10.1186/s12903-022-02332-w.

DOI:10.1186/s12903-022-02332-w

PMID:35854304

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9297621/

Abstract

BACKGROUND

Oral squamous cell carcinoma (OSCC) is the most common cancer affecting the oral and maxillofacial region. This study aimed to investigate the role of cancer stem cells (CSCs) in angiogenesis and hypoxic response in OSCC.

METHODS

This retrospective observational study evaluated 56 cases of OSCC using dual immunohistochemistry. Octamer-binding transcription factor 3/4 (OCT3/4) marker was used to evaluate CSC activity. Glucose transporter 1 (GLUT1) marker was used to evaluate the hypoxic response and angiogenesis, while endoglin (CD105) was used to evaluate the late stage of angiogenesis and blood vessel formation.

RESULTS

Co-expression of OCT3/4 and GLUT1 was noted in 11 of 12 patients with grade III OSCC. However, we did not observe co-expression of these markers in 13 of 22 patients with grade I OSCC. Although we observed a significant correlation between co-expression of GLUT1 and OCT3/4 and tumor grade, there was no significant correlation between co-expression of OCT3/4 and CD105 and different grades of OSCC.

CONCLUSIONS

CSCs could play important roles in the initial stages of hypoxic response and angiogenesis. Our result reported that in higher grades of OSCC, GLUT1 as a first response to hypoxic situations might be a result of CSCs. Further studies are required to discover other biomarkers, their roles, and associated pathways of CSCs in OSCC.

摘要

背景

口腔鳞状细胞癌（OSCC）是最常见的口腔和颌面区域癌症。本研究旨在探讨癌症干细胞（CSC）在 OSCC 中的血管生成和低氧反应中的作用。

方法

本回顾性观察研究使用双重免疫组织化学评估了 56 例 OSCC 病例。八聚体结合转录因子 3/4（OCT3/4）标志物用于评估 CSC 活性。葡萄糖转运蛋白 1（GLUT1）标志物用于评估低氧反应和血管生成，而内皮糖蛋白（CD105）用于评估血管生成和血管形成的晚期。

结果

在 12 例 3 级 OSCC 患者中有 11 例观察到 OCT3/4 和 GLUT1 的共表达。然而，在 22 例 1 级 OSCC 患者中，我们没有观察到这些标志物的共表达。尽管我们观察到 GLUT1 和 OCT3/4 共表达与肿瘤分级之间存在显著相关性，但 OCT3/4 和 CD105 共表达与不同分级的 OSCC 之间没有显著相关性。