Takayanagi T, Kawaguchi H, Yabu Y, Itoh M, Appawu M A
J Parasitol. 1987 Apr;73(2):333-41.
The role of complement in the process of binding of trypanosomes to macrophages in the presence of specific antibody was studied. The aggregation of trypanosomes observed at the optimal antigen-antibody ratio or in the presence of excess antigen inhibited the binding. Complement caused clumped trypanosomes to dissociate, and the free trypanosomes, which were presumed to be coated with antibody that had fixed complement, readily attached to surfaces of phagocytes. Thus, complement was shown to contribute at the site of the antigen-antibody reaction to the creation of an environment suitable for the binding. It seems likely that the trypanosomes dissociated by complement adhered to C3 receptors of the macrophage. However, in the absence of complement and in regions of antibody excess, free trypanosomes also attached to phagocytes. Thus phagocytes may also have receptors for the Fc portion of aggregated antibody. Complement activated by the alternate pathway also enhanced attachment of trypanosomes to phagocytes, but the effect was not as rapid as it was when complement was activated by classical means.
研究了补体在特异性抗体存在下锥虫与巨噬细胞结合过程中的作用。在最佳抗原 - 抗体比例或存在过量抗原时观察到的锥虫聚集抑制了结合。补体使聚集的锥虫解离，游离的锥虫，推测其表面包被有固定了补体的抗体，易于附着到吞噬细胞表面。因此，补体在抗原 - 抗体反应部位有助于创造一个适合结合的环境。似乎由补体解离的锥虫粘附到巨噬细胞的C3受体上。然而，在没有补体且抗体过量的区域，游离的锥虫也能附着到吞噬细胞上。因此，吞噬细胞可能也有聚集抗体Fc部分的受体。通过替代途径激活的补体也增强了锥虫与吞噬细胞的附着，但效果不如通过经典途径激活补体时迅速。
