Huang Lifu, Shao Wenfei, Wang Xiaohong, Li Feiping, Mao Weijun
Department of Urology, Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, Linhai City, Zhejiang Province, China.
Enze Hospital, Taizhou Enze Medical Center (Group), Taizhou City, Zhejiang Province, China.
Histol Histopathol. 2023 Jan;38(1):65-72. doi: 10.14670/HH-18-496. Epub 2022 Jul 20.
With the change of people's lifestyle in recent years, bladder carcinoma has been the second leading cause of death for men. Nevertheless, surgical results of bladder carcinoma are unsatisfying with recurrence and distant metastasis. Therefore, it is urgent to find a new target for bladder carcinoma treatment.
The protein and mRNA expression levels of TBX3 in bladder carcinoma tissue samples and cells were tested using western blot and qRT-PCR assays, respectively. Cancer stem cells (CSCs) were separated with immunomagnetic beads. Expression levels of cell stemness-associated proteins CD44, CD24 and ESA in T24 CSCs and T24 cells were detected by western blot assay. Cell self-renewal ability was detected by stem cell sphere formation assay. CCK-8 and colony formation assays examined cell viability and proliferation. Cell apoptotic level was examined by flow cytometry.
Elevated TBX3 expression in bladder carcinoma stimulated cell proliferation and inhibited cell apoptosis. Stemness-related proteins and TBX3 were highly expressed in T24 CSCs relative to those in normal bladder carcinoma cells. In addition, TBX3 promoted stem cell self-renewal and inhibited cell apoptosis. Finally, qRT-PCR, western blot and cell sphere formation assays revealed that the potential role of TGF-β1 in the regulation of TBX3.
TBX3, mediated by TGF-β1, can promote bladder carcinoma cell proliferation, inhibit apoptosis, and enhance cell stemness. Hence, TBX3 is a potential target to stem cells of bladder carcinoma.
近年来，随着人们生活方式的改变，膀胱癌已成为男性第二大死因。然而，膀胱癌的手术治疗结果并不理想，存在复发和远处转移的问题。因此，迫切需要寻找新的膀胱癌治疗靶点。
分别采用蛋白质免疫印迹法和qRT-PCR检测膀胱癌组织样本和细胞中TBX3的蛋白质和mRNA表达水平。用免疫磁珠分离癌症干细胞（CSCs）。通过蛋白质免疫印迹法检测T24 CSCs和T24细胞中细胞干性相关蛋白CD44、CD24和ESA的表达水平。通过干细胞球形成试验检测细胞自我更新能力。CCK-8和集落形成试验检测细胞活力和增殖情况。通过流式细胞术检测细胞凋亡水平。
膀胱癌中TBX3表达升高促进细胞增殖并抑制细胞凋亡。相对于正常膀胱癌细胞，T24 CSCs中干性相关蛋白和TBX3高表达。此外，TBX3促进干细胞自我更新并抑制细胞凋亡。最后，qRT-PCR、蛋白质免疫印迹法和细胞球形成试验揭示了TGF-β1在调节TBX3中的潜在作用。
由TGF-β1介导的TBX3可促进膀胱癌细胞增殖、抑制凋亡并增强细胞干性。因此，TBX3是膀胱癌干细胞的一个潜在靶点。
