Institute of Medicinal and Pharmaceutical Chemistry, Technische Universitaet Braunschweig, Germany.
Natural and Medical Sciences Research Center, University of Nizwa, PO Box 33, Birkat Al Mauz, Nizwa 616, Oman.
Methods Appl Fluoresc. 2022 Jul 28;10(4). doi: 10.1088/2050-6120/ac82a6.
Thepanel of technologies to address biomolecular interactions are in play, however microscale thermophoresis is continuously increasing in use to represent a key player in this arena. This review highlights the usefulness of microscale thermophoresis in the determination of molecular and biomolecular affinity interactions. This work reviews the literature from January 2016 to January 2022 about microscale thermophoresis. It gives a summarized overview about both the state-of the art and the development in the field of microscale thermophoresis. The principle of microscale thermophoresis is also described supported with self-created illustrations. Moreover, some recent advances are mentioned that showing application of the technique in investigating biomolecular interactions in different fields. Finally, advantages as well as drawbacks of the technique in comparison with other competing techniques are summarized.
用于解决生物分子相互作用的技术层出不穷，然而，微尺度热泳技术的应用不断增加，成为该领域的关键技术之一。本文重点介绍了微尺度热泳技术在确定分子和生物分子亲和力相互作用方面的应用。本文综述了 2016 年 1 月至 2022 年 1 月期间有关微尺度热泳技术的文献。它概述了微尺度热泳技术的最新进展和发展现状。文中还描述了微尺度热泳技术的原理，并配以自行创建的插图。此外，还提到了一些最新进展，展示了该技术在不同领域研究生物分子相互作用的应用。最后，总结了该技术与其他竞争技术相比的优缺点。
