Department of Stomatology, 425700Central Hospital Affiliated to Shandong First Medical University, Jinan, China.
Department of Prosthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, 66555Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, Jinan, China.
J Biomater Appl. 2022 Nov;37(5):850-858. doi: 10.1177/08853282221116855. Epub 2022 Jul 20.
Carbon dots (CDs) are one of the carbon-based materials with remarkable optical properties, good water dispersibility and high biocompatibility. However, few studies have emphasized the intrinsic photodynamic and anticancer properties of CDs. Herein, we used CDs as photosensitizers to explore their photodynamic therapy (PDT) effect on oral squamous cell carcinoma (OSCC) cells. The obtained CDs had an effective cellular internalization capacity and possessed good biocompatibility in both CAL-27 and UM1 cells. After irradiation, the CDs showed obvious photodynamic effects on the test cells, which were confirmed by apoptosis analysis. In addition, the CDs exhibited excellent intracellular ROS generation under irradiation, and the subsequent induced cell death may be related to a lysosome-associated pathway.
碳点（CDs）是具有显著光学性质、良好水分散性和高生物相容性的碳基材料之一。然而，很少有研究强调 CDs 的内在光动力和抗癌特性。在这里，我们使用 CDs 作为光敏剂来探索它们对口腔鳞状细胞癌（OSCC）细胞的光动力治疗（PDT）效果。所得 CDs 具有有效的细胞内化能力，并在 CAL-27 和 UM1 细胞中具有良好的生物相容性。照射后，CDs 在测试细胞上表现出明显的光动力效应，这通过凋亡分析得到了证实。此外，CDs 在照射下表现出优异的细胞内 ROS 生成能力，随后诱导的细胞死亡可能与溶酶体相关途径有关。
