Al-Balqa Applied University, Faculty of Science, Department of Medical Laboratory Sciences, Al-Salt, Jordan.
Braz J Biol. 2022 Jul 15;84:e260029. doi: 10.1590/1519-6984.260029. eCollection 2022.
Asphodelus fistulosus (A. fistulosus) is a wild plant grows in Jordan. Traditionally, it is used to treat different medical conditions and diseases such as respiratory ailments, against burns and dermatomucosal infections.This study aims to find out the effects of A. fistulosus aqueous and ethanolic crude extracts on Staphylococcus aureus(S. aureus) as gram positive bacteria and Escherichia coli (E. coli) as gram negative bacteria and to investigate which one will be affected either by aqueous and/or ethanolic crude extracts of A. fistulosus shooting parts that were collected from Jerash in the north of Jordan. Agar well diffusion method was used to evaluate the antibacterial activity of the crude extracts. In addition, MIC (minimum inhibitory concentration) as well as MBC (minimum bactericidal concentration) were determined against both types of bacteria. The results showed that flower aqueous extract of A. fistulosus was very effective against E. coli (20.0 ± 0.50) mm and caused a (14.0 ± 0.50) mm inhibition to S. aureus. The ethanolic extract of stem was very effective cauesed a (19.0 ± 0.50) mm inhibition in both bacterial species. Respectively, both S. aureus and E. coli were inhibited by ethanolic and aqueous extracts (mixture1 and mixture2) (15.0 ± 0.00 mm and 10.5 ± 0.50 mm). The highest antimbacterial activity was observed for the leaves aqueous extract against E.coli (0.06120 mg/mL). The obtained MIC values from A. fistulosus parts extracts demonstrated antibacterial activity ranged between 7.606 and 0.06120 mg/mL. The highest antimicrobial activity was recorded in the leaves aqueous extract against E. coli.The MBC value of stem aqueous extract was 5.00 mg/mL against both S. aureus and E. coli. On the other hand, ethanolic and aqueous extracts of the leaves gave MBC values 5.00 mg/mL, and 0.156 mg/mL, respectively, against E. coli.Based on the results of this study, it can be concluded that there is good inhibitory effect of aqueous and ethanolic of A. fistulosus shooting parts extracts on growth of E. coli and S. aureus. Adding to that, stem ethanolic extract has the most effective against S. aureus while aqueous extract of flower has the most effective against E. coli.So, it is recommended to have further future studies on the A. fistulosus shooting parts crude extract bioactive components and the mechanism of how these constituents affect these types of bacteria.
阿福花（A. fistulosus）是一种野生植物，生长在约旦。传统上，它被用于治疗各种医疗条件和疾病，如呼吸道疾病、烧伤和皮肤粘膜感染。本研究旨在研究阿福花（A. fistulosus）水提物和醇提物对金黄色葡萄球菌（S. aureus）和大肠杆菌（E. coli）的影响，以确定来自约旦北部杰拉什（Jerash）的阿福花shooting 部分的水提物和/或醇提物会影响哪一种细菌。琼脂扩散法用于评估粗提取物的抗菌活性。此外，还测定了最小抑菌浓度（MIC）和最小杀菌浓度（MBC）对这两种细菌的影响。结果表明，阿福花的花水提物对大肠杆菌（20.0 ± 0.50）mm 非常有效，并对金黄色葡萄球菌（14.0 ± 0.50）mm 产生抑制作用。茎的醇提物对两种细菌均有很强的抑制作用，分别为（19.0 ± 0.50）mm。阿福花的水提物和醇提物混合物 1 和混合物 2（15.0 ± 0.00 mm 和 10.5 ± 0.50 mm）对金黄色葡萄球菌和大肠杆菌均有抑制作用。阿福花叶水提物对大肠杆菌（0.06120 mg/mL）的抗菌活性最高。从阿福花各部位提取物中得到的 MIC 值表明，抗菌活性范围在 7.606 至 0.06120 mg/mL 之间。叶水提物对大肠杆菌的抗菌活性最强。茎水提物对金黄色葡萄球菌和大肠杆菌的 MBC 值均为 5.00 mg/mL。另一方面，叶的水提物和醇提物的 MBC 值分别为 5.00 mg/mL 和 0.156 mg/mL，对大肠杆菌有效。根据本研究的结果，可以得出结论，阿福花 shooting 部分的水提物和醇提物对大肠杆菌和金黄色葡萄球菌的生长有很好的抑制作用。此外，茎的醇提物对金黄色葡萄球菌最有效，而花的水提物对大肠杆菌最有效。因此，建议对阿福花 shooting 部分粗提取物的生物活性成分及其影响这些类型细菌的机制进行进一步的未来研究。
