Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México.
Doctorado en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Tlaxcala, México.
Gynecol Endocrinol. 2022 Sep;38(9):748-753. doi: 10.1080/09513590.2022.2099830. Epub 2022 Jul 21.
: To assess the relative expression of the G-protein coupled estrogen receptor (GPER) in the bulbospongiosus (Bsm) and pubococcygeus (Pcm) muscles in control, ovariectomized (OVX), and OVX with estradiol benzoate supplementation (OVX + EB) rabbits.: We used tissues from C, 1-month OVX, and OVX plus 15-day EB implanted (OVX + EB) groups. The GPER expression was evaluated by Western blot and immunohistochemistry for both Bsm and Pcm. : Both muscles showed a GPER immunoreactivity in blood vessels, inside myofibers next to myonuclei, and in polymorphonuclear cells. Four-week ovariectomy did not modify the GPER expression in the Bsm and Pcm, but two-week estradiol benzoate increased it in the latter muscle alone.: We demonstrated that the Bsm and Pcm of female rabbits express GPER. High serum estradiol levels elevate GPER relative expression in the Pcm alone. The present study supports the remarkable estrogen sensitivity of the Pcm.
: 评估 G 蛋白偶联雌激素受体（GPER）在对照、去卵巢（OVX）和去卵巢加苯甲酸雌二醇补充（OVX+EB）兔子的球海绵体肌（Bsm）和耻骨尾骨肌（Pcm）中的相对表达。: 我们使用 C 组、1 个月 OVX 组和 OVX 加 15 天 EB 植入（OVX+EB）组的组织。通过 Western blot 和免疫组织化学评估 Bsm 和 Pcm 中的 GPER 表达。: 两种肌肉在血管、靠近肌细胞核的肌纤维内和多形核细胞中均显示出 GPER 免疫反应性。四周卵巢切除术未改变 Bsm 和 Pcm 中的 GPER 表达，但两周苯甲酸雌二醇仅增加后者肌肉中的 GPER 表达。: 我们证明了雌性兔子的 Bsm 和 Pcm 表达 GPER。高血清雌二醇水平增加了 Pcm 中 GPER 的相对表达。本研究支持 Pcm 的显著雌激素敏感性。
