Laboratory of Reproduction Biology, Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran.
Biopreserv Biobank. 2023 Jun;21(3):233-241. doi: 10.1089/bio.2021.0145. Epub 2022 Jul 20.
Highlights Using cysteine and purslane extracts in extenders improved significantly the post-thaw sperm characteristics. Sperm viability, DNA integrity, and mitochondrial activity demonstrate an improvement in post-thaw sperm. Malondialdehyde production was decreased based on the positive effects of treated extenders. The obtained results demonstrate that supplementation of 50 μg/mL of purslane methanolic extract with cysteine to freezing extenders was significantly superior compared with other treatments.
在稀释液中使用半胱氨酸和马齿苋提取物可显著改善解冻后精子的特性。精子活力、DNA 完整性和线粒体活性均显示解冻后精子得到改善。丙二醛的产生减少,这是由于处理后的稀释液有积极作用。所得结果表明,与其他处理方法相比,在冷冻稀释液中添加 50μg/ml 的马齿苋甲醇提取物和半胱氨酸具有明显优势。