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黄腐酸对山羊精子的影响。

Effects of fulvic acids on goat sperm.

作者信息

Xiao Yu, Wu Zhengmu, Wang Min

机构信息

Reproductive Medical Center,the International Peace Maternity and Child Health Hospital of China Welfare Institute,910 Hengshan Road,Shanghai,200030,China.

出版信息

Zygote. 2018 Jun;26(3):220-223. doi: 10.1017/S0967199418000126. Epub 2018 Jun 28.

Abstract

SummaryThe effects of adding fulvic acids (FAs) to semen extenders on the quality parameters of frozen-thawed goat buck spermatozoa remain undetermined. Buck semen samples collected from six mature goat bucks once a week were diluted with Tris-egg yolk-based extenders. The diluted semen samples were supplemented with FAs (0.2, 0.4 and 0.6%, w/w), cryopreserved, and evaluated for sperm-quality parameters. Addition of FAs to the extender increased progressive motility, acrosome integrity, membrane integrity, and superoxide dismutase and catalase activities and decreased percentage abnormality and sperm malondialdehyde level compared with the control group. However, excessive FA addition (>0.4%, w/w) to semen extenders did not improve the efficiency. The results indicated that FAs could be a promising cryoprotectant for goat buck sperm.

摘要

摘要

向精液稀释液中添加黄腐酸(FAs)对冻融后的山羊精液质量参数的影响尚未确定。每周从六只成年公山羊采集一次精液样本,用基于Tris - 蛋黄的稀释液进行稀释。将稀释后的精液样本添加FAs(0.2%、0.4%和0.6%,w/w),进行冷冻保存,并评估精子质量参数。与对照组相比,向稀释液中添加FAs可提高精子的前向运动能力、顶体完整性、膜完整性以及超氧化物歧化酶和过氧化氢酶活性,并降低精子异常率和丙二醛水平。然而,向精液稀释液中添加过量的FAs(>0.4%,w/w)并不能提高效果。结果表明,FAs可能是一种有前景的山羊精液冷冻保护剂。

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