Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Trends Biotechnol. 2023 Jan;41(1):120-133. doi: 10.1016/j.tibtech.2022.06.006. Epub 2022 Jul 18.
Enzymes have essential roles in catalyzing biological reactions and maintaining metabolic systems. Many in vitro enzymatic bioassays have been developed for use in industrial and research fields, such as cell biology, enzyme engineering, drug screening, and biofuel production. Of note, many of these require the use of high-throughput platforms. Although the microtiter plate remains the standard for high-throughput enzymatic bioassays, microfluidic arrays and droplet microfluidics represent emerging methods. Each has seen significant advances and offers distinct advantages; however, drawbacks in key performance metrics, including reagent consumption, reaction manipulation, reaction recovery, real-time measurement, concentration gradient range, and multiplexity, remain. Herein, we compare recent high-throughput platforms using the aforementioned metrics as criteria and provide insights into remaining challenges and future research trends.
酶在催化生物反应和维持代谢系统方面起着至关重要的作用。许多体外酶生物测定法已经开发出来，用于工业和研究领域，如细胞生物学、酶工程、药物筛选和生物燃料生产。值得注意的是，其中许多需要使用高通量平台。尽管微孔板仍然是高通量酶生物测定的标准，但微流控阵列和液滴微流控技术是新兴的方法。每种方法都取得了重大进展，并具有独特的优势；然而，在关键性能指标方面仍存在缺陷，包括试剂消耗、反应操作、反应回收、实时测量、浓度梯度范围和多重性。在此，我们根据上述标准比较了最近的高通量平台，并深入探讨了仍存在的挑战和未来的研究趋势。
