Fox J G, Niemi S M, Ackerman J, Murphy J C
Lab Anim Sci. 1987 Feb;37(1):72-5.
An outbreak of Corynebacterium kutscheri pneumonia occurred in a colony of laboratory rats. Diagnostic methods compared on a prospective basis included (1) an enzyme-linked immunosorbent assay (ELISA) for serum antibody to C. kutscheri; (2) C. kutscheri isolation from retrograde nasal wash, cervical lymph nodes, tracheal wash, lung homogenate; and (3) histopathology. C. kutscheri was isolated from one or more of the cultured sites from suspected cases, but no individual rat yielded C. kutscheri from all of the sites. The presence of histological lung lesions was the most frequent indicator of infection (8 of 9 suspected cases), followed by isolation of C. kutscheri from lung homogenate (5 of 6), ELISA for serum antibody (6 of 8), and isolation of C. kutscheri from cervical lymph node (4 of 8). Isolation of C. kutscheri from nasal wash (1 of 9) was the least sensitive indicator of infection. ELISA antibody was not detected in rats which had normal lungs and did not harbor C. kutscheri. It was concluded that ELISA provides noninvasive means for detecting infection and cervical lymph node culture increase the potential for successful isolation of the agent in C. kutscheri infected rats.
在一群实验大鼠中发生了库氏棒状杆菌肺炎疫情。前瞻性比较的诊断方法包括：（1）检测血清中抗库氏棒状杆菌抗体的酶联免疫吸附测定（ELISA）；（2）从逆行鼻腔冲洗液、颈部淋巴结、气管冲洗液、肺匀浆中分离库氏棒状杆菌；（3）组织病理学检查。从疑似病例的一个或多个培养部位分离出了库氏棒状杆菌，但没有一只大鼠在所有部位都分离出该菌。肺部组织学病变是最常见的感染指标（9例疑似病例中有8例），其次是从肺匀浆中分离出库氏棒状杆菌（6例中有5例）、血清抗体ELISA检测（8例中有6例）以及从颈部淋巴结中分离出库氏棒状杆菌（8例中有4例）。从鼻腔冲洗液中分离出库氏棒状杆菌（9例中有1例）是最不敏感的感染指标。在肺部正常且未携带库氏棒状杆菌的大鼠中未检测到ELISA抗体。得出的结论是，ELISA提供了检测感染的非侵入性方法，而颈部淋巴结培养增加了在感染库氏棒状杆菌的大鼠中成功分离病原体的可能性。
