Short C E, Harvey R C, Fisher F E, Cunningham J N, Rose D M, Gelbfish J, Weiss E, Grossi E
Lab Anim Sci. 1987 Feb;37(1):94-9.
The purpose of this study was to determine the factors influencing successful experimental cardiopulmonary bypass studies using pulsatile flow perfusion and the medications and methodology necessary to produce successful bypass in calves. In six calves showing no cardiopulmonary pathology prior to bypass procedures, successful anesthesia and surgical intervention was accomplished. Animals were maintained on 5 hours of pulsatile flow bypass perfusion. Successful recovery from the procedures was accomplished. In two calves with pre-existing pulmonary pathology, anesthetic and surgical intervention was accomplished with the utilization of extensive anesthetic management and cardiac supportive medications until the animals could be initiated into 5 hours of pulsatile flow bypass perfusion, in spite of major pulmonary dysfunction. In these two animals, attempts to resuscitate upon termination of pulsatile flow perfusion were unsuccessful due to pre-existing excessive lesions in the lungs. This study shows a contrast between complete success of a pulsatile flow system in normal subjects versus the ultimate failure in experimental animals with pre-existing pulmonary pathology. The inability of experimental calves with a diseased lung to resume spontaneous cardiopulmonary function after the challenges of thoracic intervention indicates the unsuitability of animals with marked pre-existing pulmonary disease status for use in cardiopulmonary bypass studies.
本研究的目的是确定影响使用搏动流灌注进行成功的实验性体外循环研究的因素，以及在小牛中实现成功体外循环所需的药物和方法。在六只在体外循环手术前无心肺病理状况的小牛中，成功完成了麻醉和手术干预。动物接受了5小时的搏动流旁路灌注。手术过程成功恢复。在两只先前存在肺部病理状况的小牛中，尽管存在严重的肺功能障碍，但通过广泛的麻醉管理和心脏支持药物的使用，成功完成了麻醉和手术干预，直到动物能够开始5小时的搏动流旁路灌注。在这两只动物中，由于肺部先前存在过多病变，搏动流灌注终止后复苏尝试未成功。这项研究显示了搏动流系统在正常受试者中完全成功与在先前存在肺部病理状况的实验动物中最终失败之间的对比。患有疾病的肺部的实验小牛在接受胸部干预挑战后无法恢复自主心肺功能，这表明存在明显先前肺部疾病状态的动物不适合用于体外循环研究。
