Department of Gene Technology, Science for Life Laboratory, Royal Institute of Technology, Stockholm, Sweden.
Methods Mol Biol. 2022;2532:75-94. doi: 10.1007/978-1-0716-2497-5_5.
Targeted chromosome conformation capture (HiCap) is an experimental method for detecting spatial interactions of genomic features such as promoters and/or enhancers. The protocol first describes the design of sequence capture probes. After that, it provides details on the chromosome conformation capture adapted for next-generation sequencing (Hi-C). Finally, the methodology for coupling Hi-C with sequence capture technology is described.
靶向染色体构象捕获（HiCap）是一种用于检测基因组特征（如启动子和/或增强子）空间相互作用的实验方法。该方案首先描述了序列捕获探针的设计。之后，提供了适用于下一代测序（Hi-C）的染色体构象捕获的详细信息。最后，描述了将 Hi-C 与序列捕获技术相结合的方法学。
