Department of Bioscience and Biotechnology, Fukui Prefectural University, Eiheiji, Fukui, 910-1195, Japan.
Anal Sci. 2022 Oct;38(10):1333-1337. doi: 10.1007/s44211-022-00163-x. Epub 2022 Jul 22.
In this paper, a sensitive determination method for polyanion using a glucose oxidase (GOx) enzymatic reaction with ferricyanide ion is described. We previously reported that the GOx enzymatic reaction was significantly promoted by a cationic polymer of ε-poly-L-lysine (εPL), and the enzymatic reaction could be utilized for the determination of εPL. Generally, polycation stoichiometrically forms polyion complex with polyanion. Thus, it is expected that the promotion effect of εPL on the enzymatic reaction is interfered by polyanion, and the enzymatic reaction is also applicable to the determination of polyanion. Predictably, the promotion effect of εPL was stoichiometrically interfered by polyanions, such as polyvinyl sulfate and polyacrylate, and the interference effect allowed for the determination of the polyanions. The detection limit of polyanion was estimated to be ~ 0.3 μeq L. As a preliminary application, the proposed method was applied to the determination of anionic polymer of heparin in a human plasma.
本文描述了一种使用葡萄糖氧化酶（GOx）与铁氰化钾离子的酶反应来检测多阴离子的灵敏测定方法。我们之前曾报道过，ε-聚-L-赖氨酸（εPL）的阳离子聚合物显著促进了 GOx 酶反应，并且该酶反应可用于测定 εPL。通常情况下，聚阳离子与多阴离子按化学计量形成聚离子复合物。因此，可以预期 εPL 对酶反应的促进作用会受到多阴离子的干扰，并且该酶反应也适用于多阴离子的测定。可以预见的是，εPL 的促进作用会受到多阴离子（如聚乙烯硫酸盐和聚丙烯酸）的化学计量干扰，并且这种干扰效应可用于多阴离子的测定。多阴离子的检测限估计为~0.3 μeq L。作为初步应用，该方法被应用于人血浆中肝素阴离子聚合物的测定。
