Rodrigo-Torres Lidia, María Landete José, Huedo Pol, Peirotén Ángela, Langa Susana, Rodríguez-Minguez Eva, Medina Margarita, Arahal David R, Aznar Rosa, Arqués Juan L
Department of Microbiology and Ecology, University of Valencia, Burjassot 46100, Valencia, Spain; Spanish Type Culture Collection (CECT), University of Valencia, Paterna 46980, Valencia, Spain.
Departamento Tecnología de Alimentos, INIA-CSIC, Carretera de La Coruña Km 7, 28040 Madrid, Spain.
Gene. 2022 Oct 5;840:146743. doi: 10.1016/j.gene.2022.146743. Epub 2022 Jul 20.
Lacticaseibacillus paracasei INIA P272 and Lacticaseibacillus rhamnosus INIA P344, isolated from breast-fed infants, are two promising bacterial strains for their use in functional foods according to their demonstrated probiotic and technological characteristics. To better understand their probiotic characteristics and evaluate their safety, here we report the draft genome sequences of both strains as well as the analysis of their genetical content. The draft genomes of L. paracasei INIA P272 and L. rhamnosus INIA P344 comprise 3.01 and 3.26 Mb, a total of 2994 and 3166 genes and a GC content of 46.27 % and 46.56 %, respectively. Genomic safety was assessed following the EFSA guidelines: the identification of both strains was confirmed through Average Nucleotide Identity, and the absence of virulence, pathogenic and antibiotic resistance genes was demonstrated. The genome stability analysis revealed the presence of plasmids and phage regions in both genomes, however, CRISPR sequences and other mechanisms to fight against phage infections were encoded. The probiotic abilities of both strains were supported by the presence of genes for the synthesis of SCFA, genes involved in resistance to acid and bile salts or a thiamine production cluster. Moreover, the encoded exopolysaccharide biosynthesis genes could provide additional protection against the deleterious gastrointestinal conditions, besides which, playing a key role in adherence and coaggregation of pathogenic bacteria together with the high number of adhesion proteins and domains encoded by both genomes. Additionally, the bacteriocin cluster genes found in both strains, could provide an advantageous ability to compete against pathogenic bacteria. This genomic study supports the probiotic characteristics described previously for these two strains and satisfies the safety requirements to be used in food products.
从母乳喂养婴儿中分离出的副干酪乳杆菌INIA P272和鼠李糖乳杆菌INIA P344，根据其已证明的益生菌和技术特性，是两种有前景的用于功能性食品的细菌菌株。为了更好地了解它们的益生菌特性并评估其安全性，在此我们报告这两种菌株的基因组草图序列以及对其遗传内容的分析。副干酪乳杆菌INIA P272和鼠李糖乳杆菌INIA P344的基因组草图分别包含3.01和3.26 Mb，共有2994和3166个基因，GC含量分别为46.27%和46.56%。按照欧洲食品安全局（EFSA）的指南评估了基因组安全性：通过平均核苷酸同一性确认了两种菌株的身份，并证明不存在毒力、致病和抗生素抗性基因。基因组稳定性分析显示，两种基因组中均存在质粒和噬菌体区域，然而，编码了CRISPR序列和其他抵抗噬菌体感染的机制。两种菌株的益生菌能力得到了短链脂肪酸合成基因、参与抗酸和抗胆盐的基因或硫胺素产生簇的支持。此外，编码的胞外多糖生物合成基因除了在致病细菌的黏附和共聚中发挥关键作用以及两种基因组编码的大量黏附蛋白和结构域外，还可以提供针对有害胃肠道状况的额外保护。此外，在两种菌株中发现的细菌素簇基因，可以提供与致病细菌竞争的有利能力。这项基因组研究支持了先前描述的这两种菌株的益生菌特性，并满足了用于食品的安全要求。
