Chemical Engineering Laboratory, Faculty of Sciences and Center for Advanced Scientific Research - Centro de Investigaciones Científicas Avanzadas (CICA), BIOENGIN Group, University of La Coruña, E-15008-La Coruña, Spain.
Chemical Engineering Laboratory, Faculty of Sciences and Center for Advanced Scientific Research - Centro de Investigaciones Científicas Avanzadas (CICA), BIOENGIN Group, University of La Coruña, E-15008-La Coruña, Spain.
Bioresour Technol. 2022 Sep;360:127649. doi: 10.1016/j.biortech.2022.127649. Epub 2022 Jul 19.
Volatile fatty acids (VFAs) can be considered as low-cost carbon substrates for lipid accumulation by oleaginous yeasts. This study demonstrates that a common mixture of VFAs, typically obtained from the anaerobic fermentation of C1-gases by some acetogenic bacteria, can be used in a second aerobic fermentation with the yeast Yarrowia lipolytica to obtain lipids as precursors of biodiesel. In the batch experiments, the preference of Yarrowia lipolytica W29 for acetic acid over butyric and caproic acids was demonstrated, with the highest consumption rate reaching 0.664 g/L·h. In the bioreactor experiments, the amount initial biomass inoculated, as well as the initial acid concentration, were found to have a significant influence on the process. Though the lipid content was relatively low, it can be optimized and further improved. Oleic, linoleic and palmitic acids accounted for about 80 % of the fatty acids in the lipids, which makes them suitable for biodiesel.
挥发性脂肪酸（VFAs）可以被视为产油酵母积累油脂的低成本碳源。本研究表明，一种常见的 VFAs 混合物，通常是由某些产乙酸细菌对 C1 气体的厌氧发酵得到的，可以在第二次有氧发酵中与酵母解脂耶氏酵母（Yarrowia lipolytica）一起使用，以获得生物柴油的前体脂质。在分批实验中，证明了解脂耶氏酵母 W29 对乙酸的偏好超过丁酸和己酸，最高消耗速率达到 0.664 g/L·h。在生物反应器实验中，发现初始接种生物量和初始酸浓度对该过程有显著影响。尽管脂质含量相对较低，但可以对其进行优化和进一步改进。油酸、亚油酸和棕榈酸占脂质中脂肪酸的 80%左右，这使得它们适合用于生物柴油。
