Department of Environmental Engineering, Federal University of Lavras (Universidade Federal de Lavras, UFLA), Lavras, Minas Gerais, Brazil.
Bioresour Technol. 2022 Oct;361:127654. doi: 10.1016/j.biortech.2022.127654. Epub 2022 Jul 20.
Co-digestion is a strategy that may prevent microbial inhibition during the anaerobic treatment of sugarcane vinasse, a substrate with highly biodegradable organic load, acidity, and toxic compound levels (e.g., sulfates, phenols). In this context, this study evaluated the microbial dynamics and methanogenic potential throughout the co-digestion of sugarcane vinasse and secondary effluent from the dairy industry in a mesophilic lab-scale upflow anaerobic sludge blanket (UASB) reactor. Periodic next-generation sequencing (NGS) analyses revealed an increase in the relative abundance of the phylum Euryarchaeota (+8.6 % after inoculation), predominating hydrogenotrophic methanogens (Methanobacterium and Methanobrevibacter) at the end of the operation. Moreover, the average methane yield was 221 mLCH gCOD, with 69 % of organic matter removal. These results evidenced a progressive acclimation of the anaerobic microbial community to the substrate and a stable operation. Therefore, the proposed experiment demonstrates energy advantages for the agro-industrial sector by implementing a similar but full-scale treatment plant.
共消化是一种策略，可防止在厌氧处理甘蔗废醪（一种具有高可生物降解有机负荷、酸度和有毒化合物水平（例如硫酸盐、酚类）的底物）时出现微生物抑制。在这种情况下，本研究在中温实验室规模上的上流厌氧污泥床（UASB）反应器中，评估了甘蔗废醪和乳制品工业二级出水共消化过程中的微生物动态和产甲烷潜力。定期进行下一代测序（NGS）分析显示，古菌门的相对丰度增加（接种后增加了 8.6%），在操作结束时，产氢甲烷菌（甲烷杆菌属和甲烷短杆菌属）占优势。此外，平均甲烷产量为 221 mLCH gCOD，有机物去除率为 69%。这些结果证明了厌氧微生物群落对底物的逐渐适应和稳定运行。因此，通过实施类似但全规模的处理厂，该实验证明了该方法在农业-工业部门的能源优势。
