Deanery of Biomedical Science, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh, EH8 9XD, UK.
Immunology and Stem Cell Biology, Aravind Medical Research Foundation, Madurai, 625020, India.
Sci Rep. 2022 Jul 22;12(1):12573. doi: 10.1038/s41598-022-16768-1.
There is intense worldwide effort in generating kidney organoids from pluripotent stem cells, for research, for disease modelling and, perhaps, for making transplantable organs. Organoids generated from pluripotent stem cells (PSC) possess accurate micro-anatomy, but they lack higher-organization. This is a problem, especially for transplantation, as such organoids will not be able to perform their physiological functions. In this study, we develop a method for generating murine kidney organoids with improved higher-order structure, through stages using chimaeras of ex-fetu and PSC-derived cells to a system that works entirely from embryonic stem cells. These organoids have nephrons organised around a single ureteric bud tree and also make vessels, with the endothelial network approaching podocytes.
全世界正在努力从多能干细胞中生成肾类器官，用于研究、疾病建模，也许还用于制造可移植器官。多能干细胞（PSC）生成的类器官具有准确的微观解剖结构，但缺乏更高层次的组织。这是一个问题，特别是对于移植来说，因为这样的类器官将无法发挥其生理功能。在这项研究中，我们通过使用来自胎儿和 PSC 衍生细胞的嵌合体的阶段，开发了一种生成具有改进的更高阶结构的小鼠肾类器官的方法，发展到完全从胚胎干细胞工作的系统。这些类器官具有围绕单个输尿管芽树组织的肾单位，并且还生成血管，内皮网络接近足细胞。
