Laboratoire de recherche, Centre National de Recherche et de Formation sur le Paludisme (CNRFP), Ouagadougou, Burkina Faso.
Laboratoire d'Entomologie Fondamentale et Appliquée, Université Joseph Ki-ZERBO, Ouagadougou, Burkina Faso.
Med Vet Entomol. 2022 Sep;36(3):309-319. doi: 10.1111/mve.12602. Epub 2022 Jul 23.
The response to recent dengue outbreaks in Burkina Faso was insecticide-based, despite poor knowledge of the vector population's susceptibility to the insecticides used. Here, we report on the susceptibility to the main insecticide classes and identify important underlying mechanisms in Aedes aegypti populations in Ouagadougou and Banfora, in 2019 and 2020. Wild Ae. aegypti were tested as adults in WHO bioassays and then screened in real time melting curve qPCR analyses to genotype the F1534C, V1016I, and V410L Aedes kdr mutations. Ae. aegypti showed moderate resistance to 0.1% bendiocarb (80-95% survival post-exposure), 0.8% Malathion (60-100%), 0.21% pirimiphos-methyl (75% - 97%), and high resistance to 0.03% deltamethrin (20-70%). PBO pre-exposure partially restored pyrethroid susceptibility. Genotyping detected high frequency of 1534C allele (0.92) and moderate 1016I (0.1-0.32). The V410L mutation was detected in Burkina Faso for the first time (frequency 0.1-0.36). Mosquitoes surviving 4 h exposure to 0.03% deltamethrin had significantly higher frequencies of the F1534C mutation than dead mosquitoes (0.70 vs. 0.96, p < 0.0001) and mosquitoes surviving 2 - 4 h exposure had a significantly reduced life span. Ae. aegypti from Burkina Faso are resistant to multiple insecticide classes with multiple mechanisms involved, demonstrating the essential role of insecticide resistance monitoring within national dengue control programmes.
尽管对所用杀虫剂的媒介种群敏感性了解甚少，但布基纳法索最近登革热疫情的应对措施仍是基于杀虫剂。在这里，我们报告了 2019 年和 2020 年在瓦加杜古和邦福拉的埃及伊蚊种群对主要杀虫剂类别的敏感性，并确定了重要的潜在机制。野生埃及伊蚊成虫在世界卫生组织生物测定中进行了测试，然后在实时熔解曲线 qPCR 分析中进行了筛选，以对 F1534C、V1016I 和 V410L 埃及 kdr 突变进行基因分型。埃及伊蚊对 0.1%苯氧威（暴露后 80-95%存活）、0.8%马拉硫磷（60-100%）、0.21%吡虫啉（75%-97%）表现出中等抗性，对 0.03%溴氰菊酯表现出高度抗性（20-70%）。PBO 预暴露部分恢复了拟除虫菊酯的敏感性。基因分型检测到 1534C 等位基因的高频（0.92）和中度 1016I（0.1-0.32）。V410L 突变在布基纳法索首次被检测到（频率 0.1-0.36）。在 0.03%溴氰菊酯暴露 4 小时后存活的蚊子比死亡的蚊子（0.70 比 0.96，p<0.0001）和暴露 2-4 小时后存活的蚊子的 F1534C 突变频率显著更高。来自布基纳法索的埃及伊蚊对多种杀虫剂类别的抗性涉及多种机制，表明在国家登革热控制计划中进行杀虫剂抗性监测至关重要。
