College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China; Tianjin Key Laboratory of Food Biotechnology, Tianjin 300134, China.
College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China.
Food Chem. 2022 Dec 1;396:133716. doi: 10.1016/j.foodchem.2022.133716. Epub 2022 Jul 16.
The low allowable limit of triazine herbicides (THs) in rice makes it imperative to develop novel sample pretreatment methods for extraction and preconcentration of THs. Herein, a phosphoric acid activated biochar (PBC) was prepared and modified by chitosan (CS). For THs with different polarities, CS-PBC with multiple interaction sites exhibited satisfactory chemisorption. On this basis, a CS-PBC-based pipette tip-solid phase extraction (PT-SPE) was developed combined with HPLC to extract THs from rice. Low limits of detection (1.41-3.35 ng g), satisfactory linearity (0.01-2.00 μg g, R > 0.9974) and recoveries (96.13-116.25 %) were obtained with acceptable inter-day and intra-day precision (RSD ≤ 13.60 %). CS-PBC showed superior performance to three commercial single-mode adsorbents and comparable results to a hydrophilic-lipophilic balance adsorbent. The study explored the feasibility of PT-SPE for extracting THs from rice and broadened the application of plant biochar as an environmentally-friendly matrix in food sample pretreatment.
三嗪类除草剂（THs）在水稻中的允许限量低，因此必须开发新型的样品预处理方法来提取和浓缩 THs。本文制备了一种经壳聚糖（CS）改性的磷酸活化生物炭（PBC）。对于具有不同极性的 THs，具有多个相互作用位点的 CS-PBC 表现出了令人满意的化学吸附作用。在此基础上，结合高效液相色谱法，开发了一种基于 CS-PBC 的移液管尖端固相萃取（PT-SPE）方法，用于从水稻中提取 THs。该方法的检测限低（1.41-3.35ng g），线性良好（0.01-2.00μg g，R>0.9974），回收率（96.13-116.25%）令人满意，日内和日间精密度（RSD≤13.60%）可接受。CS-PBC 的性能优于三种商业单模式吸附剂，与亲水-亲脂平衡吸附剂的结果相当。该研究探索了 PT-SPE 从水稻中提取 THs 的可行性，并拓宽了植物生物炭作为环境友好型基质在食品样品预处理中的应用。
