Division of Public Health, Infectious Diseases, and Occupational Medicine, Mayo Clinic, Rochester, MN, USA.
Division of Clinical Microbiology, Mayo Clinic, Rochester, MN, USA.
Diagn Microbiol Infect Dis. 2022 Oct;104(2):115759. doi: 10.1016/j.diagmicrobio.2022.115759. Epub 2022 Jul 1.
Use of the Biolog OmniLog® phenotyping system for antibiotic susceptibility testing (AST) was evaluated using 51 clinical isolates of Staphylococcus aureus. MIC testing by broth microdilution was compared to results generated using the OmniLog® system for oxacillin, daptomycin, vancomycin, gentamicin, linezolid, and tetracycline. There was >90% essential and categorical agreement between methods for all antibiotics, except gentamicin, which had 83.6% essential agreement, although very major errors occurred with linezolid (n = 3) and daptomycin (n = 1). Precision was satisfactory, with 5 triplicate measurements in agreement. A quantitative threshold allowed automated interpretation of MICs yielding results comparable to manual interpretation; oxacillin, gentamicin, and tetracycline resistance could be identified at a median of 7.13, 5.25, and 7.25 hours, respectively. Limitations include the small number of isolates, and especially resistant isolates tested, and the focus on a single species. Overall, the OmniLog® system was a precise method for AST of S. aureus, although accuracy was imperfect.
使用 Biolog OmniLog®表型系统对 51 株金黄色葡萄球菌临床分离株进行抗生素药敏试验（AST）评估。肉汤微量稀释法 MIC 检测与 OmniLog®系统用于检测苯唑西林、达托霉素、万古霉素、庆大霉素、利奈唑胺和四环素的结果进行比较。除庆大霉素外，所有抗生素的方法之间均具有>90%的重要和分类一致性，庆大霉素的重要一致性为 83.6%，尽管利奈唑胺（n=3）和达托霉素（n=1）出现了非常大的错误。精密度令人满意，5 次重复测量结果一致。定量阈值允许自动解释 MIC，产生与手动解释相当的结果；苯唑西林、庆大霉素和四环素耐药性的中位检测时间分别为 7.13、5.25 和 7.25 小时。局限性包括测试的分离株数量较少，尤其是耐药分离株较少，以及专注于单一物种。总的来说，OmniLog®系统是一种用于金黄色葡萄球菌 AST 的精确方法，尽管准确性不完美。
