He Qiburi, Gong Gaowa, Wan Tingting, Hu He, Yu Peng
Key Laboratory of Industrial Fermentation Microbiology of Ministry of Education, Tianjin Key Laboratory of Industry Microbiology, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China.
Inner Mongolia Academy of Agricultural and Animal Husbandry Science, Hohhot, China.
Front Microbiol. 2023 Sep 7;14:1241462. doi: 10.3389/fmicb.2023.1241462. eCollection 2023.
() has been extensively utilized as human food or animal feed additives. With its ability to support heterologous gene expression, proves to be a valuable platform for the synthesis of proteins and metabolites that possess both high nutritional and economic value. However, there remains a dearth of research focused on the characteristics of through genomic, transcriptomic and metabolic approaches.
With the aim of unraveling the molecular mechanism and genetic basis governing the biological process of , we embarked on a sequencing endeavor to acquire comprehensive sequence data. In addition, an integrated transcriptomic and metabolic phenotype analysis was performed to compare the wild-type (WT) with a genetically engineered strain of that harbors the heterologous gene (RCT).
is a protein rich in methionine found in the endosperm of maize. The integrated analysis of transcriptomic and metabolic phenotypes uncovered significant metabolic diversity between the WT and RCT . A total of 252 differentially expressed genes were identified, primarily associated with ribosome function, peroxisome activity, arginine and proline metabolism, carbon metabolism, and fatty acid degradation. In the experimental setup using PM1, PM2, and PM4 plates, a total of 284 growth conditions were tested. A comparison between the WT and RCT demonstrated significant increases in the utilization of certain carbon source substrates by RCT. Gelatin and glycogen were found to be significantly utilized to a greater extent by RCT compared to WT. Additionally, in terms of sulfur source substrates, RCT exhibited significantly increased utilization of O-Phospho-L-Tyrosine and L-Methionine Sulfone when compared to WT.
The introduction of gene into may lead to significant changes in the metabolic substrates and metabolic pathways, but does not weaken the activity of the strain. Our study provides new insights into the transcriptomic and metabolic characteristics of the genetically engineered strain harboring gene, which has the potential to advance the utilization of as an efficient protein feed in agricultural applications.
（）已被广泛用作人类食品或动物饲料添加剂。凭借其支持异源基因表达的能力，被证明是合成具有高营养价值和经济价值的蛋白质和代谢物的宝贵平台。然而，通过基因组、转录组和代谢方法对的特性进行的研究仍然匮乏。
为了解析调控生物过程的分子机制和遗传基础，我们开展了测序工作以获取全面的序列数据。此外，进行了综合转录组和代谢表型分析，以比较野生型（WT）和携带异源基因（RCT）的基因工程菌株。
是一种在玉米胚乳中发现的富含蛋氨酸的蛋白质。转录组和代谢表型的综合分析揭示了WT和RCT之间显著的代谢多样性。共鉴定出252个差异表达基因，主要与核糖体功能、过氧化物酶体活性、精氨酸和脯氨酸代谢、碳代谢以及脂肪酸降解有关。在使用PM1、PM2和PM4平板的实验设置中，总共测试了284种生长条件。WT和RCT的比较表明，RCT对某些碳源底物的利用率显著增加。与WT相比，发现RCT对明胶和糖原的利用程度明显更高。此外，在硫源底物方面，与WT相比，RCT对O-磷酸-L-酪氨酸和L-蛋氨酸砜的利用率显著增加。
将基因引入可能会导致代谢底物和代谢途径发生显著变化，但不会削弱菌株的活性。我们的研究为携带基因的基因工程菌株的转录组和代谢特征提供了新的见解，这有可能推动在农业应用中作为高效蛋白质饲料的利用。
