Gómez-Pérez Luis S, Moraga Nelson, Ah-Hen Kong Shun, Rodríguez Angela, Vega-Gálvez Antonio
Department of Food Engineering, Universidad de La Serena, Avda. Raúl Bitrán 1305, La Serena, Chile.
Department of Mechanical Engineering, Universidad de La Serena, Benavente 980, La Serena, Chile.
J Food Sci Technol. 2022 Aug;59(8):3093-3101. doi: 10.1007/s13197-022-05416-1. Epub 2022 Mar 15.
In this study the dietary fibre (DF) of fresh and processed murta berries was characterised with respect to bioactive and antioxidant properties. DF content was determined by three different methods (AOAC, dialysis membrane and indigestible fraction methods) comparing their effectiveness. The antioxidant activity of phenolic compounds (PC) in purée, fruit leather and isolated DF of murta berries was evaluated by DPPH and ORAC assays. Total phenolics and flavonoids linked to DF were determined by colorimetric tests, while PC profiles in the respective murta products were identified by HPLC. DF concentration was > 50 g/100 g dm, fluctuating between 52.03 ± 1.32 and 57.18 ± 0.61 g/100 g dm, exhibiting simultaneously high antioxidant activity, with ORAC values of 38,190 ± 661 and 26,425 ± 1399 µmol Trolox equivalent/100 g dm in the isolated DF of fresh murta berries and processed murta fruit leather respectively, which enabled a classification as antioxidant dietary fibre. Total phenolics and flavonoids linked to DF retained a high concentration in the fruit leather, implying a favourable effect of processing. Gallic acid and pyrogallol were found associated to DF, at a level of 34.03 ± 1.17 and 631.07 ± 22.10 mg/100 g dm in the fresh berries respectively, which conferred to murta berries a functional property not yet reported.
The online version contains supplementary material available at 10.1007/s13197-022-05416-1.
在本研究中,对新鲜和加工后的穆尔塔浆果的膳食纤维(DF)的生物活性和抗氧化特性进行了表征。通过三种不同方法(美国官方分析化学师协会方法、透析膜法和不可消化部分法)测定DF含量,并比较它们的有效性。通过DPPH和ORAC试验评估了穆尔塔浆果泥、果脯和分离出的DF中酚类化合物(PC)的抗氧化活性。通过比色法测定与DF相关的总酚和黄酮类化合物,同时通过高效液相色谱法鉴定各穆尔塔产品中的PC谱。DF浓度>50 g/100 g干物质,在52.03±1.32和57.18±0.61 g/100 g干物质之间波动,同时表现出高抗氧化活性,新鲜穆尔塔浆果分离出的DF和加工后的穆尔塔果脯的ORAC值分别为38,190±661和26,425±1399 μmol Trolox当量/100 g干物质,这使其能够被归类为抗氧化膳食纤维。与DF相关的总酚和黄酮类化合物在果脯中保持高浓度,这意味着加工具有有利影响。发现没食子酸和连苯三酚与DF相关,在新鲜浆果中的含量分别为34.03±1.17和631.07±22.10 mg/100 g干物质,这赋予了穆尔塔浆果一种尚未报道的功能特性。
在线版本包含可在10.1007/s13197-022-05416-1获取的补充材料。