College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.
Sindh Institute of Animal Health, Karachi, Pakistan.
Biomed Res Int. 2022 Jul 14;2022:6209047. doi: 10.1155/2022/6209047. eCollection 2022.
Three hundred sixty ( = 360) broiler chickens were equally divided into control (C) and thiram (T) groups. Furthermore, the C and T groups were dividedinto 8-, 9-, 11-, and 13-day-old chickens.
Clinically, it was observed that broiler chickens of group T had abnormal posture, gait, and lameness, and histopathological results revealed dead and abnormal chondrocytes of T group on day 6. Real-time qPCR results showed that HDAC1, MTA1, H4, and PCNA genes were significantly expressed ( < 0.05). HDAC1 was upregulated on days 1, 2, 4, and 6 ( < 0.01); MTA1 was upregulated on days 1 and 2 ( < 0.01); H4 was upregulated on days 2 and 4 ( < 0.01), and PCNA was downregulated on days 1, 2, and 4 ( < 0.01). Furthermore, IHC results of HDAC1 protein were significantly ( < 0.01) expressed in proliferative zone of day 1 and hypertrophic zone of day 6. MTA1 protein was significantly ( < 0.01) expressed on days 1, 2, and 6 in all zones, except prehypertrophic zone of day 2.
In conclusion, the mRNA expressions of HDAC1, MTA1, H4, and PCNA were differentially expressed in the chondrocytes of thiram-induced TD chickens. HDAC1 and MTA1 protein expression found involved and responsible in the abnormal chondrocytes' proliferation of broiler chicken.
将 360 只肉鸡平均分为对照组（C）和福美双（T）组。进一步将 C 组和 T 组分为 8 日龄、9 日龄、11 日龄和 13 日龄鸡。
临床观察发现 T 组肉鸡出现姿势、步态和跛行异常，组织病理学结果显示 T 组肉鸡在第 6 天出现死亡和异常软骨细胞。实时 qPCR 结果显示，HDAC1、MTA1、H4 和 PCNA 基因表达显著（<0.05）。HDAC1 在第 1、2、4 和 6 天上调（<0.01）；MTA1 在第 1 和 2 天上调（<0.01）；H4 在第 2 和 4 天上调（<0.01），PCNA 在第 1、2 和 4 天下调（<0.01）。此外，HDAC1 蛋白的 IHC 结果在第 1 天的增殖区和第 6 天的肥大区表达显著（<0.01）。MTA1 蛋白在第 1、2 和 6 天在所有区域表达显著（<0.01），除了第 2 天的预肥大区。
综上所述，福美双诱导的 TD 鸡软骨细胞中 HDAC1、MTA1、H4 和 PCNA 的 mRNA 表达存在差异。发现 HDAC1 和 MTA1 蛋白表达参与并负责肉鸡异常软骨细胞的增殖。
